Embryonic lethal mutation in mice induced by retrovirus insertion into the α1(I) collagen gene

Angelika Schnieke, Klaus Harbers, Rudolf Jaenisch

Research output: Contribution to journalArticlepeer-review

282 Scopus citations

Abstract

Experimental insertion of a retrovirus into the germ line of mice has resulted in an embryonic recessive lethal mutation. Integration of the proviral genome occurred at the 5′ end of the α1(I) collagen gene, leading to a complete transcriptional block. Developmental arrest of embryos homozygous at the mutated allele coincides with high expression of the gene in normal embryogenesis. Insertion mutagenesis by retroviruses may offer a general approach to the identification and isolation of genes which are transcriptionally active during mammalian development.

Original languageEnglish
Pages (from-to)315-320
Number of pages6
JournalNature
Volume304
Issue number5924
DOIs
StatePublished - 1983
Externally publishedYes

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