ELISpot assay as a sensitive tool to detect cellular immunity following influenza vaccination in kidney transplant recipients

Monika Lindemann, Oliver Witzke, Peter Lütkes, Melanie Fiedler, Ernst Kreuzfelder, Thomas Philipp, Michael Roggendorf, Hans Grosse-Wilde

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Enhanced cellular immunity following influenza vaccination has been undetectable in kidney transplant recipients so far. Protection from influenza is dependent on cellular and humoral immunity. Aim of the study was to investigate immune responses before and after vaccination with influenza A and B antigens in 65 kidney transplant recipients. A significant increase in proliferative responses was only observed towards influenza B (P < 0.0001) by lymphocyte transformation test. The enzyme-linked immunospot (ELISpot) assay was more sensitive and detected significant, 3- to 5-fold increases (P < 0.0001) in interferon-γ secretion using influenza A and B antigens. Furthermore, influenza antibody titers increased significantly (P < 0.0001). At month 1 post-vaccination 85% of patients displayed specific cellular, and 95% or 92% humoral immunity against influenza A and B, respectively. Thus, applying the sensitive ELISpot assay, influenza-specific cellular immunity could be detected for the first time in kidney transplant recipients after vaccination.

Original languageEnglish
Pages (from-to)342-348
Number of pages7
JournalClinical Immunology
Volume120
Issue number3
DOIs
StatePublished - Sep 2006
Externally publishedYes

Keywords

  • Cellular immunity
  • ELISpot
  • Influenza vaccination
  • Kidney transplantation
  • Lymphocyte transformation test

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