Elimination of point streaking on silver stained two‐dimensional gels by addition of iodoacetamide to the equilibration buffer

Angelika Görg, Wilhelm Postel, Johann Weser, Siegfried Günther, John R. Strahler, Samir M. Hanash, Luke Somerlot

Research output: Contribution to journalArticlepeer-review

241 Scopus citations

Abstract

Silver staining artifacts, unrelated to protein, such as horizontal lines and vertical micro or point streaking on two‐dimensional gels with carrier ampholytes or immobilized pH gradients in the first dimension are efficiently eliminated by adding iodoacetamide to the equilibration buffer of the first‐dimensional gel.

Original languageEnglish
Pages (from-to)122-124
Number of pages3
JournalELECTROPHORESIS
Volume8
Issue number2
DOIs
StatePublished - 1987

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