Elimination of hepatitis B virus surface antigen and appearance of neutralizing antibodies in chronically infected patients without viral clearance

Z. H. Zhang; L. Li; X. P. Zhao; D. Glebe; C. M. Bremer; Z. M. Zhang; Y. J. Tian; B. J. Wang; Y. Yang; W. Gerlich; M. Roggendorf; X. Li; M. Lu; D. L. Yang

doi:10.1111/j.1365-2893.2010.01322.x

Elimination of hepatitis B virus surface antigen and appearance of neutralizing antibodies in chronically infected patients without viral clearance

Z. H. Zhang
, L. Li
, X. P. Zhao
, D. Glebe
, C. M. Bremer
, Z. M. Zhang
, Y. J. Tian
, B. J. Wang
, Y. Yang
, W. Gerlich
, M. Roggendorf
, X. Li
, M. Lu
, D. L. Yang

Huazhong University of Science and Technology
Anhui Medical University
Justus-Liebig-Universität Gießen
University Hospital of Essen
Tongji Medical College

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Summary. Seroconversion from hepatitis B surface antigen (HBsAg) to antibodies against HBsAg (anti-HBs) usually indicates resolution of hepatitis B virus (HBV) infection. Here, two HBV-infected patients with seroconversion to anti-HBs were found to be persistently positive for HBeAg and HBV DNA. Immunohistology of liver biopsies confirmed the expression of HBV proteins in the liver of one patient. The neutralizing ability of anti-HBs in patient sera was demonstrated by blocking HBV infection of primary tupaia hepatocytes. Analysis of the HBsAg-encoding region of HBV isolates from patients indicated the coexistence of heterogeneous HBV genomes in patients. The majority of recombinant variant HBsAg was reactive in HBsAg assays and was able to bind to anti-HBs. Circulating immune complexes (CIC) of HBsAg in patient sera could be detected by polyethylene glycol precipitation and trypsin digestion. Thus, neutralizing anti-HBs may appear in chronic HBV carriers for long periods but does not necessarily lead to complete viral clearance.

Original language	English
Pages (from-to)	424-433
Number of pages	10
Journal	Journal of Viral Hepatitis
Volume	18
Issue number	6
DOIs	https://doi.org/10.1111/j.1365-2893.2010.01322.x
State	Published - Jun 2011
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

hepatitis B surface antigen
hepatitis B virus
persistent HBV infection
seroconversion
surface antibody

Access to Document

10.1111/j.1365-2893.2010.01322.x

Cite this

Zhang, Z. H., Li, L., Zhao, X. P., Glebe, D., Bremer, C. M., Zhang, Z. M., Tian, Y. J., Wang, B. J., Yang, Y., Gerlich, W., Roggendorf, M., Li, X., Lu, M., & Yang, D. L. (2011). Elimination of hepatitis B virus surface antigen and appearance of neutralizing antibodies in chronically infected patients without viral clearance. Journal of Viral Hepatitis, 18(6), 424-433. https://doi.org/10.1111/j.1365-2893.2010.01322.x

@article{8401e891b87b4188a338b34a8384b429,

title = "Elimination of hepatitis B virus surface antigen and appearance of neutralizing antibodies in chronically infected patients without viral clearance",

abstract = "Summary. Seroconversion from hepatitis B surface antigen (HBsAg) to antibodies against HBsAg (anti-HBs) usually indicates resolution of hepatitis B virus (HBV) infection. Here, two HBV-infected patients with seroconversion to anti-HBs were found to be persistently positive for HBeAg and HBV DNA. Immunohistology of liver biopsies confirmed the expression of HBV proteins in the liver of one patient. The neutralizing ability of anti-HBs in patient sera was demonstrated by blocking HBV infection of primary tupaia hepatocytes. Analysis of the HBsAg-encoding region of HBV isolates from patients indicated the coexistence of heterogeneous HBV genomes in patients. The majority of recombinant variant HBsAg was reactive in HBsAg assays and was able to bind to anti-HBs. Circulating immune complexes (CIC) of HBsAg in patient sera could be detected by polyethylene glycol precipitation and trypsin digestion. Thus, neutralizing anti-HBs may appear in chronic HBV carriers for long periods but does not necessarily lead to complete viral clearance.",

keywords = "hepatitis B surface antigen, hepatitis B virus, persistent HBV infection, seroconversion, surface antibody",

author = "Zhang, \{Z. H.\} and L. Li and Zhao, \{X. P.\} and D. Glebe and Bremer, \{C. M.\} and Zhang, \{Z. M.\} and Tian, \{Y. J.\} and Wang, \{B. J.\} and Y. Yang and W. Gerlich and M. Roggendorf and X. Li and M. Lu and Yang, \{D. L.\}",

year = "2011",

month = jun,

doi = "10.1111/j.1365-2893.2010.01322.x",

language = "English",

volume = "18",

pages = "424--433",

journal = "Journal of Viral Hepatitis",

issn = "1352-0504",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "6",

}

Zhang, ZH, Li, L, Zhao, XP, Glebe, D, Bremer, CM, Zhang, ZM, Tian, YJ, Wang, BJ, Yang, Y, Gerlich, W, Roggendorf, M, Li, X, Lu, M & Yang, DL 2011, 'Elimination of hepatitis B virus surface antigen and appearance of neutralizing antibodies in chronically infected patients without viral clearance', Journal of Viral Hepatitis, vol. 18, no. 6, pp. 424-433. https://doi.org/10.1111/j.1365-2893.2010.01322.x

TY - JOUR

T1 - Elimination of hepatitis B virus surface antigen and appearance of neutralizing antibodies in chronically infected patients without viral clearance

AU - Zhang, Z. H.

AU - Li, L.

AU - Zhao, X. P.

AU - Glebe, D.

AU - Bremer, C. M.

AU - Zhang, Z. M.

AU - Tian, Y. J.

AU - Wang, B. J.

AU - Yang, Y.

AU - Gerlich, W.

AU - Roggendorf, M.

AU - Li, X.

AU - Lu, M.

AU - Yang, D. L.

PY - 2011/6

Y1 - 2011/6

N2 - Summary. Seroconversion from hepatitis B surface antigen (HBsAg) to antibodies against HBsAg (anti-HBs) usually indicates resolution of hepatitis B virus (HBV) infection. Here, two HBV-infected patients with seroconversion to anti-HBs were found to be persistently positive for HBeAg and HBV DNA. Immunohistology of liver biopsies confirmed the expression of HBV proteins in the liver of one patient. The neutralizing ability of anti-HBs in patient sera was demonstrated by blocking HBV infection of primary tupaia hepatocytes. Analysis of the HBsAg-encoding region of HBV isolates from patients indicated the coexistence of heterogeneous HBV genomes in patients. The majority of recombinant variant HBsAg was reactive in HBsAg assays and was able to bind to anti-HBs. Circulating immune complexes (CIC) of HBsAg in patient sera could be detected by polyethylene glycol precipitation and trypsin digestion. Thus, neutralizing anti-HBs may appear in chronic HBV carriers for long periods but does not necessarily lead to complete viral clearance.

AB - Summary. Seroconversion from hepatitis B surface antigen (HBsAg) to antibodies against HBsAg (anti-HBs) usually indicates resolution of hepatitis B virus (HBV) infection. Here, two HBV-infected patients with seroconversion to anti-HBs were found to be persistently positive for HBeAg and HBV DNA. Immunohistology of liver biopsies confirmed the expression of HBV proteins in the liver of one patient. The neutralizing ability of anti-HBs in patient sera was demonstrated by blocking HBV infection of primary tupaia hepatocytes. Analysis of the HBsAg-encoding region of HBV isolates from patients indicated the coexistence of heterogeneous HBV genomes in patients. The majority of recombinant variant HBsAg was reactive in HBsAg assays and was able to bind to anti-HBs. Circulating immune complexes (CIC) of HBsAg in patient sera could be detected by polyethylene glycol precipitation and trypsin digestion. Thus, neutralizing anti-HBs may appear in chronic HBV carriers for long periods but does not necessarily lead to complete viral clearance.

KW - hepatitis B surface antigen

KW - hepatitis B virus

KW - persistent HBV infection

KW - seroconversion

KW - surface antibody

UR - https://www.scopus.com/pages/publications/79955444867

U2 - 10.1111/j.1365-2893.2010.01322.x

DO - 10.1111/j.1365-2893.2010.01322.x

M3 - Article

C2 - 20819150

AN - SCOPUS:79955444867

SN - 1352-0504

VL - 18

SP - 424

EP - 433

JO - Journal of Viral Hepatitis

JF - Journal of Viral Hepatitis

IS - 6

ER -

Elimination of hepatitis B virus surface antigen and appearance of neutralizing antibodies in chronically infected patients without viral clearance

Abstract

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this