Elicitation of volatile compounds in photomixotrophic cell culture of Petroselinum crispum

Gerold Reil, Ralf G. Berger

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Photomixotrophic cells of Petroselinum crispum accumulated > 500 mg chlorophyll per kg wet weight and grew well in a broad range of phytoeffector conditions. Autoclaved fungal cells were lethal for photoheterotrophic cells, but induced in photomixotrophic cells the formation of volatile n-alkanes, phthalides, coumarins, and elemicine. Most of the compounds elicited reached a concentration maximum between 20 and 30 h after addition of the mycelium, whereas the group of n-alkanes increased steadily during the 90 h monitored. Maximum concentrations were: 12 mg of graveolone, 1 mg of bergapten, 0.5 mg of sedanenolide, and 0.5 mg of n-etradecane per 1 nutrient medium. A dose/effect relationship was found; 10 to 25 g of fungal wet weight per 1 culture medium resulted in maximum accumulation of volatiles. The formation of volatiles by photomixotrophic in vitro cells is discussed as an integral part of plant responses to ecological stress.

Original languageEnglish
Pages (from-to)131-136
Number of pages6
JournalPlant Cell, Tissue and Organ Culture
Volume46
Issue number2
DOIs
StatePublished - 1996

Keywords

  • elicitation
  • furocoumarins
  • Petroselinum crispum
  • photomixotrophy
  • phthalides
  • volatile compounds

Fingerprint

Dive into the research topics of 'Elicitation of volatile compounds in photomixotrophic cell culture of Petroselinum crispum'. Together they form a unique fingerprint.

Cite this