TY - GEN
T1 - Electrophysiological recordings of cortico-striatal network activity in microfluidic-mea-hybrid system
AU - Stevanović, Jelena
AU - Zobel, Kathrin
AU - Wolfrum, Bernhard
AU - Offenhäusser, Andreas
N1 - Publisher Copyright:
© 2019 CBMS-0001.
PY - 2019
Y1 - 2019
N2 - In our work, we report on the development of a microelectrode(MEA)-microfluidic hybrid system, capable of studying electrophysiology of dissociated neuronal cultures. We present electrophysiological recordings of cortico-striatal network connected by different microchannel lengths of asymmetric axon-diode shape. These asymmetric microchannel shapes serve as extracellular signal amplifiers of directed axonal growth. The average recorded peak to peak action potentials on adjacent electrodes varies between 0.4 mV and 0.6 mV in DIV14 culture, increasing up to 1.8 mV and 2.4 mV after DIV20, respectively. We conclude that the most common signal shape is triphasic, while other less common shapes as pentaphasic ´w´ shape are also present.
AB - In our work, we report on the development of a microelectrode(MEA)-microfluidic hybrid system, capable of studying electrophysiology of dissociated neuronal cultures. We present electrophysiological recordings of cortico-striatal network connected by different microchannel lengths of asymmetric axon-diode shape. These asymmetric microchannel shapes serve as extracellular signal amplifiers of directed axonal growth. The average recorded peak to peak action potentials on adjacent electrodes varies between 0.4 mV and 0.6 mV in DIV14 culture, increasing up to 1.8 mV and 2.4 mV after DIV20, respectively. We conclude that the most common signal shape is triphasic, while other less common shapes as pentaphasic ´w´ shape are also present.
KW - Cortico-Striatal Network
KW - Extracellular Recordings
KW - Microelectrode Arrays
KW - Microfluidics
UR - http://www.scopus.com/inward/record.url?scp=85094947522&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85094947522
T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
SP - 789
EP - 790
BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PB - Chemical and Biological Microsystems Society
T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Y2 - 27 October 2019 through 31 October 2019
ER -