Electrochemical microfluidic biosensor for the protein-based quantification of β-lactams

R. Bruch, C. Chatelle, A. Kling, C. Dincer, W. Weber, G. Urban

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this work, we present a novel approach for the point-of-care testing (POCT) of β-lactam antibiotics on a miniaturized electrochemical microfluidic platform. Herein, a highly sensitive, lowcost and versatile biosensor is combined with an enzyme linked assay (ELA) using the penicillin binding protein 3 (PBP-3) for the fast and facile detection of s-lactams from very low sample volumes (580 nl). The demonstration of the system applicability is successfully shown by measuring different piperacillin concentrations in spiked human blood plasma with an inter-assay coefficient of variation (CV) below 15 % and a limit of detection (LOD) of 8.34 ng ml-1.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages1667-1668
Number of pages2
ISBN (Electronic)9780979806490
StatePublished - 2016
Externally publishedYes
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/TerritoryIreland
CityDublin
Period9/10/1613/10/16

Keywords

  • Antibiotics
  • Electrochemical biosensor
  • Penicillin binding protein 3
  • Point-of-care

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