Electrochemical detection of food-spoiling bacteria using interdigitated platinum microelectrodes

Olga Fysun, Alexander Schmitt, Peter Thomas Auernhammer, Johannes Rauschnabel, Horst Christian Langowski

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The fast and non-destructive detection of bacterial attachment on food contact surfaces is important for the prevention of the unwanted formation of biofilms. Biofilms constitute a protected growth mode that allows bacteria to survive even in hostile environments. Therefore, the fast detection of bacterial attachment may be an effective strategy for biofilm control. In this study cyclic voltammetry (CV)was used to detect Bacillus subtilis ssp. subtilis, Paenibacillus polymyxa, Pseudomonas fragi attachment on interdigitated microelectrodes. The differences in current between the uncolonized sterile microelectrodes and the microelectrodes after bacterial attachment were determined. In addition, the surface coverage of microelectrodes was visualized using microscopy techniques. The results showed that the cyclic voltammetry in combination with interdigitated platinum microelectrodes can be used to detect bacterial biofilms.

Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalJournal of Microbiological Methods
Volume161
DOIs
StatePublished - Jun 2019

Keywords

  • Bacillus subtilis ssp. subtilis
  • Bacterial biofilms
  • Cyclic voltammetry
  • Interdigitated microelectrodes
  • Paenibacillus polymyxa
  • Pseudomonas fragi

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