TY - JOUR
T1 - Electrochemical detection of food-spoiling bacteria using interdigitated platinum microelectrodes
AU - Fysun, Olga
AU - Schmitt, Alexander
AU - Auernhammer, Peter Thomas
AU - Rauschnabel, Johannes
AU - Langowski, Horst Christian
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/6
Y1 - 2019/6
N2 - The fast and non-destructive detection of bacterial attachment on food contact surfaces is important for the prevention of the unwanted formation of biofilms. Biofilms constitute a protected growth mode that allows bacteria to survive even in hostile environments. Therefore, the fast detection of bacterial attachment may be an effective strategy for biofilm control. In this study cyclic voltammetry (CV)was used to detect Bacillus subtilis ssp. subtilis, Paenibacillus polymyxa, Pseudomonas fragi attachment on interdigitated microelectrodes. The differences in current between the uncolonized sterile microelectrodes and the microelectrodes after bacterial attachment were determined. In addition, the surface coverage of microelectrodes was visualized using microscopy techniques. The results showed that the cyclic voltammetry in combination with interdigitated platinum microelectrodes can be used to detect bacterial biofilms.
AB - The fast and non-destructive detection of bacterial attachment on food contact surfaces is important for the prevention of the unwanted formation of biofilms. Biofilms constitute a protected growth mode that allows bacteria to survive even in hostile environments. Therefore, the fast detection of bacterial attachment may be an effective strategy for biofilm control. In this study cyclic voltammetry (CV)was used to detect Bacillus subtilis ssp. subtilis, Paenibacillus polymyxa, Pseudomonas fragi attachment on interdigitated microelectrodes. The differences in current between the uncolonized sterile microelectrodes and the microelectrodes after bacterial attachment were determined. In addition, the surface coverage of microelectrodes was visualized using microscopy techniques. The results showed that the cyclic voltammetry in combination with interdigitated platinum microelectrodes can be used to detect bacterial biofilms.
KW - Bacillus subtilis ssp. subtilis
KW - Bacterial biofilms
KW - Cyclic voltammetry
KW - Interdigitated microelectrodes
KW - Paenibacillus polymyxa
KW - Pseudomonas fragi
UR - http://www.scopus.com/inward/record.url?scp=85064677111&partnerID=8YFLogxK
U2 - 10.1016/j.mimet.2019.04.015
DO - 10.1016/j.mimet.2019.04.015
M3 - Article
C2 - 31022418
AN - SCOPUS:85064677111
SN - 0167-7012
VL - 161
SP - 63
EP - 70
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
ER -