Electrical and optical characterization of thrombin-induced permeability of cultured endothelial cell monolayers on semiconductor electrode arrays

H. Hillebrandt; A. Abdelghani; C. Abdelghani-Jacquin; M. Aepfelbacher; E. Sackmann

doi:10.1007/s003390100879

Electrical and optical characterization of thrombin-induced permeability of cultured endothelial cell monolayers on semiconductor electrode arrays

H. Hillebrandt
, A. Abdelghani
, C. Abdelghani-Jacquin
, M. Aepfelbacher
, E. Sackmann

Department of Bioscience

Technical University of Munich
IPEST
Ludwig-Maximilians-Universität München

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Impedance spectroscopy and phase-contrast microscopy are combined to monitor the electrical and morpho-logical properties of human umbilical vein endothelial cell monolayers. The cells were cultured on optically transparent indium-tin-oxide (ITO) semiconductor electrode arrays coated with collagen IV, and the effect of the inflammatory mediator thrombin on monolayer permeability was monitored in real time. ITO electrodes provide several advantages for these kinds of experiments, because they are optically transparent, polarizable and highly sensitive due to the absence of insulating oxide layers. A qualitative correlation between the thrombin-induced gap formation and the electrical parameters of the cell layer is established.

Original language	English
Pages (from-to)	539-546
Number of pages	8
Journal	Applied Physics A: Materials Science and Processing
Volume	73
Issue number	5
DOIs	https://doi.org/10.1007/s003390100879
State	Published - Nov 2001

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10.1007/s003390100879

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title = "Electrical and optical characterization of thrombin-induced permeability of cultured endothelial cell monolayers on semiconductor electrode arrays",

abstract = "Impedance spectroscopy and phase-contrast microscopy are combined to monitor the electrical and morpho-logical properties of human umbilical vein endothelial cell monolayers. The cells were cultured on optically transparent indium-tin-oxide (ITO) semiconductor electrode arrays coated with collagen IV, and the effect of the inflammatory mediator thrombin on monolayer permeability was monitored in real time. ITO electrodes provide several advantages for these kinds of experiments, because they are optically transparent, polarizable and highly sensitive due to the absence of insulating oxide layers. A qualitative correlation between the thrombin-induced gap formation and the electrical parameters of the cell layer is established.",

author = "H. Hillebrandt and A. Abdelghani and C. Abdelghani-Jacquin and M. Aepfelbacher and E. Sackmann",

year = "2001",

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doi = "10.1007/s003390100879",

language = "English",

volume = "73",

pages = "539--546",

journal = "Applied Physics A: Materials Science and Processing",

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Electrical and optical characterization of thrombin-induced permeability of cultured endothelial cell monolayers on semiconductor electrode arrays. / Hillebrandt, H.; Abdelghani, A.; Abdelghani-Jacquin, C. et al.
In: Applied Physics A: Materials Science and Processing, Vol. 73, No. 5, 11.2001, p. 539-546.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Electrical and optical characterization of thrombin-induced permeability of cultured endothelial cell monolayers on semiconductor electrode arrays

AU - Hillebrandt, H.

AU - Abdelghani, A.

AU - Abdelghani-Jacquin, C.

AU - Aepfelbacher, M.

AU - Sackmann, E.

PY - 2001/11

Y1 - 2001/11

N2 - Impedance spectroscopy and phase-contrast microscopy are combined to monitor the electrical and morpho-logical properties of human umbilical vein endothelial cell monolayers. The cells were cultured on optically transparent indium-tin-oxide (ITO) semiconductor electrode arrays coated with collagen IV, and the effect of the inflammatory mediator thrombin on monolayer permeability was monitored in real time. ITO electrodes provide several advantages for these kinds of experiments, because they are optically transparent, polarizable and highly sensitive due to the absence of insulating oxide layers. A qualitative correlation between the thrombin-induced gap formation and the electrical parameters of the cell layer is established.

AB - Impedance spectroscopy and phase-contrast microscopy are combined to monitor the electrical and morpho-logical properties of human umbilical vein endothelial cell monolayers. The cells were cultured on optically transparent indium-tin-oxide (ITO) semiconductor electrode arrays coated with collagen IV, and the effect of the inflammatory mediator thrombin on monolayer permeability was monitored in real time. ITO electrodes provide several advantages for these kinds of experiments, because they are optically transparent, polarizable and highly sensitive due to the absence of insulating oxide layers. A qualitative correlation between the thrombin-induced gap formation and the electrical parameters of the cell layer is established.

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U2 - 10.1007/s003390100879

DO - 10.1007/s003390100879

M3 - Article

AN - SCOPUS:0035500947

SN - 0947-8396

VL - 73

SP - 539

EP - 546

JO - Applied Physics A: Materials Science and Processing

JF - Applied Physics A: Materials Science and Processing

IS - 5

ER -

Electrical and optical characterization of thrombin-induced permeability of cultured endothelial cell monolayers on semiconductor electrode arrays

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