Electric cell-substrate impedance sensing with screen printed electrode structures

Martin Brischwein; Sigrun Herrmann; Winfried Vonau; Frank Berthold; Helmut Grothe; Elena Roza Motrescu; Bernhard Wolf

doi:10.1039/b602987f

Electric cell-substrate impedance sensing with screen printed electrode structures

Martin Brischwein
, Sigrun Herrmann
, Winfried Vonau
, Frank Berthold
, Helmut Grothe
, Elena Roza Motrescu
, Bernhard Wolf

Former organisations of Engineering and Design

Technical University of Munich
Kurt-Schwabe-Institut für Mess- und Sensortechnik e.V. Meinsberg

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Impedance sensors in thick film technology have been tested as a tool for electric cell-substrate impedance sensing. The screen printed Pt electrodes have a width of 250-400 μm. Electrodes and the surrounding ceramic chip substrate could be homogeneously grown with L-929 and Hela cells. The performance of a screen printed interdigitated electrode structure (IDES) was compared with that of thin film structures with the same layout geometry. The thick film impedance sensors allowed to correctly record the morphological response of confluent Hela cell layers to stimulation with histamine. A thick film conductivity sensor also revealed impedance values which were dependent on cell growth on the electrode surface, even at a very low frequency range of ≈1 Hz.

Original language	English
Pages (from-to)	819-822
Number of pages	4
Journal	Lab on a Chip
Volume	6
Issue number	6
DOIs	https://doi.org/10.1039/b602987f
State	Published - 2006

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abstract = "Impedance sensors in thick film technology have been tested as a tool for electric cell-substrate impedance sensing. The screen printed Pt electrodes have a width of 250-400 μm. Electrodes and the surrounding ceramic chip substrate could be homogeneously grown with L-929 and Hela cells. The performance of a screen printed interdigitated electrode structure (IDES) was compared with that of thin film structures with the same layout geometry. The thick film impedance sensors allowed to correctly record the morphological response of confluent Hela cell layers to stimulation with histamine. A thick film conductivity sensor also revealed impedance values which were dependent on cell growth on the electrode surface, even at a very low frequency range of ≈1 Hz.",

author = "Martin Brischwein and Sigrun Herrmann and Winfried Vonau and Frank Berthold and Helmut Grothe and Motrescu, \{Elena Roza\} and Bernhard Wolf",

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AU - Brischwein, Martin

AU - Herrmann, Sigrun

AU - Vonau, Winfried

AU - Berthold, Frank

AU - Grothe, Helmut

AU - Motrescu, Elena Roza

AU - Wolf, Bernhard

PY - 2006

Y1 - 2006

N2 - Impedance sensors in thick film technology have been tested as a tool for electric cell-substrate impedance sensing. The screen printed Pt electrodes have a width of 250-400 μm. Electrodes and the surrounding ceramic chip substrate could be homogeneously grown with L-929 and Hela cells. The performance of a screen printed interdigitated electrode structure (IDES) was compared with that of thin film structures with the same layout geometry. The thick film impedance sensors allowed to correctly record the morphological response of confluent Hela cell layers to stimulation with histamine. A thick film conductivity sensor also revealed impedance values which were dependent on cell growth on the electrode surface, even at a very low frequency range of ≈1 Hz.

AB - Impedance sensors in thick film technology have been tested as a tool for electric cell-substrate impedance sensing. The screen printed Pt electrodes have a width of 250-400 μm. Electrodes and the surrounding ceramic chip substrate could be homogeneously grown with L-929 and Hela cells. The performance of a screen printed interdigitated electrode structure (IDES) was compared with that of thin film structures with the same layout geometry. The thick film impedance sensors allowed to correctly record the morphological response of confluent Hela cell layers to stimulation with histamine. A thick film conductivity sensor also revealed impedance values which were dependent on cell growth on the electrode surface, even at a very low frequency range of ≈1 Hz.

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JO - Lab on a Chip

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ER -

Electric cell-substrate impedance sensing with screen printed electrode structures

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