Efficient synthesis and applications of peptides containing adenylylated tyrosine residues

Cornelis Smit; Julia Blümer; Martijn F. Eerland; Michael F. Albers; Matthias P. Müller; Roger S. Goody; Aymelt Itzen; Christian Hedberg

doi:10.1002/anie.201103203

Efficient synthesis and applications of peptides containing adenylylated tyrosine residues

Cornelis Smit, Julia Blümer, Martijn F. Eerland, Michael F. Albers, Matthias P. Müller, Roger S. Goody, Aymelt Itzen, Christian Hedberg

Max Planck Inst. fur Molec. Physiol.

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The enzyme DrrA of the human pathogen Legionella pneumophila adenylylates specifically a tyrosine of the GTPase Rab1. An efficient synthesis route using Fmoc solid phase peptide synthesis led to Tyr-adenylylated peptides and allowed the generation of mono-selective polyclonal antibodies against this post-translational modification.

Original language	English
Pages (from-to)	9200-9204
Number of pages	5
Journal	Angewandte Chemie International Edition in English
Volume	50
Issue number	39
DOIs	https://doi.org/10.1002/anie.201103203
State	Published - 19 Sep 2011
Externally published	Yes

Keywords

Rab proteins
adenylylation
antibodies
peptides
post-translational modifications

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10.1002/anie.201103203

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abstract = "The enzyme DrrA of the human pathogen Legionella pneumophila adenylylates specifically a tyrosine of the GTPase Rab1. An efficient synthesis route using Fmoc solid phase peptide synthesis led to Tyr-adenylylated peptides and allowed the generation of mono-selective polyclonal antibodies against this post-translational modification.",

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author = "Cornelis Smit and Julia Bl{\"u}mer and Eerland, {Martijn F.} and Albers, {Michael F.} and M{\"u}ller, {Matthias P.} and Goody, {Roger S.} and Aymelt Itzen and Christian Hedberg",

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AU - Blümer, Julia

AU - Eerland, Martijn F.

AU - Albers, Michael F.

AU - Müller, Matthias P.

AU - Goody, Roger S.

AU - Itzen, Aymelt

AU - Hedberg, Christian

PY - 2011/9/19

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AB - The enzyme DrrA of the human pathogen Legionella pneumophila adenylylates specifically a tyrosine of the GTPase Rab1. An efficient synthesis route using Fmoc solid phase peptide synthesis led to Tyr-adenylylated peptides and allowed the generation of mono-selective polyclonal antibodies against this post-translational modification.

KW - Rab proteins

KW - adenylylation

KW - antibodies

KW - peptides

KW - post-translational modifications

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IS - 39

ER -

Efficient synthesis and applications of peptides containing adenylylated tyrosine residues

Abstract

Keywords

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