Efficient ribosomal peptidyl transfer critically relies on the presence of the ribose 2′-OH at A2451 of 23S rRNA

Matthias D. Erlacher, Kathrin Lang, Brigitte Wotzel, Renate Rieder, Ronald Micura, Norbert Polacek

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

The ribosomal peptidyl transferase center is a ribozyme catalyzing peptide bond synthesis in all organisms. We applied a novel modified nucleoside interference approach to identify functional groups at 9 universally conserved active site residues. Owing to their immediate proximity to the chemical center, the 23S rRNA nucleosides A2451, U2506 and U2585 were of particular interest. Our study ruled out U2506 and U2585 as contributors of vital chemical groups for transpeptidation. In contrast the ribose 2′-OH of A2451 was identified as the prime ribosomal group with potential functional importance. This 2′-OH renders almost full catalytic power to the ribosome even when embedded into an active site of six neighboring 2′-deoxyribose nucleosides. These data highlight the unique functional role of the A2451 2′-OH for peptide bond synthesis among all other functional groups at the ribosomal peptidyl transferase active site.

Original languageEnglish
Pages (from-to)4453-4459
Number of pages7
JournalJournal of the American Chemical Society
Volume128
Issue number13
DOIs
StatePublished - 5 Apr 2006
Externally publishedYes

Fingerprint

Dive into the research topics of 'Efficient ribosomal peptidyl transfer critically relies on the presence of the ribose 2′-OH at A2451 of 23S rRNA'. Together they form a unique fingerprint.

Cite this