Efficient ex vivo delivery of chemically modified messenger RNA using lipofection and magnetofection

Zohreh Sadat Badieyan; Tamara Pasewald; Olga Mykhaylyk; Carsten Rudolph; Christian Plank

doi:10.1016/j.bbrc.2016.11.113

Efficient ex vivo delivery of chemically modified messenger RNA using lipofection and magnetofection

Zohreh Sadat Badieyan, Tamara Pasewald, Olga Mykhaylyk, Carsten Rudolph, Christian Plank

Institute of Molecular Immunology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Recently, chemically modified mRNA (cmRNA) therapeutics have been the subject of extensive application-oriented research in both academia and industry as a safer alternative for gene and recombinant protein therapies. However, the lack of an efficient delivery system hinders widespread application. Here we used ∼100-nm lipoplexes and magnetic lipoplexes that can protect cmRNA from RNases and efficiently deliver it into muscle and fat tissues as well as to the endothelium of the carotid artery. Establishing magnetofection for ex vivo cmRNA delivery for the first time, we suggest this method for potential enhanced and targeted delivery of cmRNA. This study introduces optimal cmRNA complexes with high ex vivo efficiency as good candidates for further in vivo cmRNA delivery.

Original language	English
Pages (from-to)	796-801
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	482
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2016.11.113
State	Published - 22 Jan 2017

Keywords

Chemically modified mRNA (cmRNA)
Lipoplex
Magnetofection
cmRNA delivery system

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10.1016/j.bbrc.2016.11.113

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abstract = "Recently, chemically modified mRNA (cmRNA) therapeutics have been the subject of extensive application-oriented research in both academia and industry as a safer alternative for gene and recombinant protein therapies. However, the lack of an efficient delivery system hinders widespread application. Here we used ∼100-nm lipoplexes and magnetic lipoplexes that can protect cmRNA from RNases and efficiently deliver it into muscle and fat tissues as well as to the endothelium of the carotid artery. Establishing magnetofection for ex vivo cmRNA delivery for the first time, we suggest this method for potential enhanced and targeted delivery of cmRNA. This study introduces optimal cmRNA complexes with high ex vivo efficiency as good candidates for further in vivo cmRNA delivery.",

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AU - Badieyan, Zohreh Sadat

AU - Pasewald, Tamara

AU - Mykhaylyk, Olga

AU - Rudolph, Carsten

AU - Plank, Christian

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AB - Recently, chemically modified mRNA (cmRNA) therapeutics have been the subject of extensive application-oriented research in both academia and industry as a safer alternative for gene and recombinant protein therapies. However, the lack of an efficient delivery system hinders widespread application. Here we used ∼100-nm lipoplexes and magnetic lipoplexes that can protect cmRNA from RNases and efficiently deliver it into muscle and fat tissues as well as to the endothelium of the carotid artery. Establishing magnetofection for ex vivo cmRNA delivery for the first time, we suggest this method for potential enhanced and targeted delivery of cmRNA. This study introduces optimal cmRNA complexes with high ex vivo efficiency as good candidates for further in vivo cmRNA delivery.

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Efficient ex vivo delivery of chemically modified messenger RNA using lipofection and magnetofection

Abstract

Keywords

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