Efficient ex vivo delivery of chemically modified messenger RNA using lipofection and magnetofection

Zohreh Sadat Badieyan, Tamara Pasewald, Olga Mykhaylyk, Carsten Rudolph, Christian Plank

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Recently, chemically modified mRNA (cmRNA) therapeutics have been the subject of extensive application-oriented research in both academia and industry as a safer alternative for gene and recombinant protein therapies. However, the lack of an efficient delivery system hinders widespread application. Here we used ∼100-nm lipoplexes and magnetic lipoplexes that can protect cmRNA from RNases and efficiently deliver it into muscle and fat tissues as well as to the endothelium of the carotid artery. Establishing magnetofection for ex vivo cmRNA delivery for the first time, we suggest this method for potential enhanced and targeted delivery of cmRNA. This study introduces optimal cmRNA complexes with high ex vivo efficiency as good candidates for further in vivo cmRNA delivery.

Original languageEnglish
Pages (from-to)796-801
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume482
Issue number4
DOIs
StatePublished - 22 Jan 2017

Keywords

  • Chemically modified mRNA (cmRNA)
  • Lipoplex
  • Magnetofection
  • cmRNA delivery system

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