Efficient and safe gene delivery to human corneal endothelium using magnetic nanoparticles

Marta Czugala; Olga Mykhaylyk; Philip Böhler; Jasmine Onderka; Björn Stork; Sebastian Wesselborg; Friedrich E. Kruse; Christian Plank; Bernhard B. Singer; Thomas A. Fuchsluger

doi:10.2217/nnm-2016-0144

Efficient and safe gene delivery to human corneal endothelium using magnetic nanoparticles

Marta Czugala, Olga Mykhaylyk, Philip Böhler, Jasmine Onderka, Björn Stork, Sebastian Wesselborg, Friedrich E. Kruse, Christian Plank, Bernhard B. Singer, Thomas A. Fuchsluger

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Aim: To develop a safe and efficient method for targeted, anti-apoptotic gene therapy of corneal endothelial cells (CECs). Materials & methods: Magnetofection (MF), a combination of lipofection with magnetic nanoparticles (MNPs; PEI-Mag2, SO-Mag5, PalD1-Mag1), was tested in human CECs and in explanted human corneas. Effects on cell viability and function were investigated. Immunocompatibility was assessed in human peripheral blood mononuclear cells. Results: Silica iron-oxide MNPs (SO-Mag5) combined with X-tremeGENE-HP achieved high transfection efficiency in human CECs and explanted human corneas, without altering cell viability or function. Magnetofection caused no immunomodulatory effects in human peripheral blood mononuclear cells. Magnetofection with anti-apoptotic P35 gene effectively blocked apoptosis in CECs. Conclusion: Magnetofection is a promising tool for gene therapy of corneal endothelial cells with potential for targeted on-site delivery.

Original language	English
Pages (from-to)	1787-1800
Number of pages	14
Journal	Nanomedicine
Volume	11
Issue number	14
DOIs	https://doi.org/10.2217/nnm-2016-0144
State	Published - Jul 2016
Externally published	Yes

Keywords

apoptosis
corneal endothelium
gene therapy
magnetic nanoparticles
magnetofection

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title = "Efficient and safe gene delivery to human corneal endothelium using magnetic nanoparticles",

abstract = "Aim: To develop a safe and efficient method for targeted, anti-apoptotic gene therapy of corneal endothelial cells (CECs). Materials & methods: Magnetofection (MF), a combination of lipofection with magnetic nanoparticles (MNPs; PEI-Mag2, SO-Mag5, PalD1-Mag1), was tested in human CECs and in explanted human corneas. Effects on cell viability and function were investigated. Immunocompatibility was assessed in human peripheral blood mononuclear cells. Results: Silica iron-oxide MNPs (SO-Mag5) combined with X-tremeGENE-HP achieved high transfection efficiency in human CECs and explanted human corneas, without altering cell viability or function. Magnetofection caused no immunomodulatory effects in human peripheral blood mononuclear cells. Magnetofection with anti-apoptotic P35 gene effectively blocked apoptosis in CECs. Conclusion: Magnetofection is a promising tool for gene therapy of corneal endothelial cells with potential for targeted on-site delivery.",

keywords = "apoptosis, corneal endothelium, gene therapy, magnetic nanoparticles, magnetofection",

author = "Marta Czugala and Olga Mykhaylyk and Philip B{\"o}hler and Jasmine Onderka and Bj{\"o}rn Stork and Sebastian Wesselborg and Kruse, {Friedrich E.} and Christian Plank and Singer, {Bernhard B.} and Fuchsluger, {Thomas A.}",

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year = "2016",

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doi = "10.2217/nnm-2016-0144",

language = "English",

volume = "11",

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T1 - Efficient and safe gene delivery to human corneal endothelium using magnetic nanoparticles

AU - Czugala, Marta

AU - Mykhaylyk, Olga

AU - Böhler, Philip

AU - Onderka, Jasmine

AU - Stork, Björn

AU - Wesselborg, Sebastian

AU - Kruse, Friedrich E.

AU - Plank, Christian

AU - Singer, Bernhard B.

AU - Fuchsluger, Thomas A.

PY - 2016/7

Y1 - 2016/7

N2 - Aim: To develop a safe and efficient method for targeted, anti-apoptotic gene therapy of corneal endothelial cells (CECs). Materials & methods: Magnetofection (MF), a combination of lipofection with magnetic nanoparticles (MNPs; PEI-Mag2, SO-Mag5, PalD1-Mag1), was tested in human CECs and in explanted human corneas. Effects on cell viability and function were investigated. Immunocompatibility was assessed in human peripheral blood mononuclear cells. Results: Silica iron-oxide MNPs (SO-Mag5) combined with X-tremeGENE-HP achieved high transfection efficiency in human CECs and explanted human corneas, without altering cell viability or function. Magnetofection caused no immunomodulatory effects in human peripheral blood mononuclear cells. Magnetofection with anti-apoptotic P35 gene effectively blocked apoptosis in CECs. Conclusion: Magnetofection is a promising tool for gene therapy of corneal endothelial cells with potential for targeted on-site delivery.

AB - Aim: To develop a safe and efficient method for targeted, anti-apoptotic gene therapy of corneal endothelial cells (CECs). Materials & methods: Magnetofection (MF), a combination of lipofection with magnetic nanoparticles (MNPs; PEI-Mag2, SO-Mag5, PalD1-Mag1), was tested in human CECs and in explanted human corneas. Effects on cell viability and function were investigated. Immunocompatibility was assessed in human peripheral blood mononuclear cells. Results: Silica iron-oxide MNPs (SO-Mag5) combined with X-tremeGENE-HP achieved high transfection efficiency in human CECs and explanted human corneas, without altering cell viability or function. Magnetofection caused no immunomodulatory effects in human peripheral blood mononuclear cells. Magnetofection with anti-apoptotic P35 gene effectively blocked apoptosis in CECs. Conclusion: Magnetofection is a promising tool for gene therapy of corneal endothelial cells with potential for targeted on-site delivery.

KW - apoptosis

KW - corneal endothelium

KW - gene therapy

KW - magnetic nanoparticles

KW - magnetofection

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JO - Nanomedicine

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ER -

Efficient and safe gene delivery to human corneal endothelium using magnetic nanoparticles

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Keywords

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