Effects of pentachlorophenol and 2,4,6-trichlorophenol on the disposition of sulfobromophthalein and respiration of isolated liver cells

R. Götz, L. R. Schwarz, H. Greim

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8 Scopus citations

Abstract

The effect of pentachlorophenol (PCP) and 2,4,6-trichlorophenol (2,4,6 T) on the disposition of the hepatodiagnostic dye, sulfobromophthalein (BSP) has been studied in isolated liver cells. PCP (4-6 μM) as well as 2,4,6-T (50-100 μM) interferes with the disposition of BSP. The main effect apparently occurs at the secretion step as both drugs severely impair the release of the glutathione conjugate of BSP into the medium. As a consequence, BSP and its conjugate accumulate in the cell. High doses of PCP did not increase the release of lactate dehydrogenase from the hepatocytes. Concentrations of the two phenols which interfere with the secretion of BSP also completely uncouple the oxidative phosphorylation of hepatocellular mitochondria. The dysfunction of liver cells described here may therefore be explained by the effect of PCP and 2,4,6-T on the energy production of the cells. The higher toxicity of PCP as compared to 2,4,6-T observed in our system corresponds well with the higher LD50 of the latter compound.

Original languageEnglish
Pages (from-to)147-155
Number of pages9
JournalArchives of Toxicology
Volume44
Issue number1-3
DOIs
StatePublished - Mar 1980
Externally publishedYes

Keywords

  • 2,4,6-Trichlorophenol
  • Energy metabolism
  • Isolated hepatocytes
  • Pentachlorophenol
  • Sulfobromophthalein

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