TY - JOUR
T1 - Effects of obestatin on energy balance and growth hormone secretion in rodents
AU - Nogueiras, Rubén
AU - Pfluger, Paul
AU - Tovar, Sulay
AU - Arnold, Myrtha
AU - Mitchell, Sharon
AU - Morris, Amanda
AU - Perez-Tilve, Diego
AU - Vázquez, Maria J.
AU - Wiedmer, Petra
AU - Castañeda, Tamara R.
AU - DiMarchi, Richard
AU - Tschöp, Matthias
AU - Schurmann, Annette
AU - Joost, Hans Georg
AU - Williams, Lynda M.
AU - Langhans, Wolfgang
AU - Diéguez, Carlos
PY - 2007
Y1 - 2007
N2 - Ghrelin stimulates food intake and adiposity and thereby increases body weight (BW) in rodents after central as well as peripheral administration. Recently, it was discovered that the gene precursor of ghrelin encoded another secreted and bioactive peptide named obestatin. First reports appeared to demonstrate that this peptide requires an amidation for its biological activity and acts through the orphan receptor, GPR-39. Obestatin was shown to have actions opposite to ghrelin on food intake, BW, and gastric emptying. In the present study, we failed to observe any effect of obestatin on food intake, BW, body composition, energy expenditure, locomotor activity, respiratory quotient, or hypothalamic neuropeptides involved in energy balance regulation. In agreement with the first report, we were unable to find any effect of obestatin on GH secretion in vivo. Moreover, we were unable to find mRNA expression of GPR-39, the putative obestatin receptor, in the hypothalamus of rats. Therefore, the results presented here do not support a role of the obestatin/GPR-39 system in the regulation of energy balance.
AB - Ghrelin stimulates food intake and adiposity and thereby increases body weight (BW) in rodents after central as well as peripheral administration. Recently, it was discovered that the gene precursor of ghrelin encoded another secreted and bioactive peptide named obestatin. First reports appeared to demonstrate that this peptide requires an amidation for its biological activity and acts through the orphan receptor, GPR-39. Obestatin was shown to have actions opposite to ghrelin on food intake, BW, and gastric emptying. In the present study, we failed to observe any effect of obestatin on food intake, BW, body composition, energy expenditure, locomotor activity, respiratory quotient, or hypothalamic neuropeptides involved in energy balance regulation. In agreement with the first report, we were unable to find any effect of obestatin on GH secretion in vivo. Moreover, we were unable to find mRNA expression of GPR-39, the putative obestatin receptor, in the hypothalamus of rats. Therefore, the results presented here do not support a role of the obestatin/GPR-39 system in the regulation of energy balance.
UR - http://www.scopus.com/inward/record.url?scp=33845911774&partnerID=8YFLogxK
U2 - 10.1210/en.2006-0915
DO - 10.1210/en.2006-0915
M3 - Article
C2 - 17008393
AN - SCOPUS:33845911774
SN - 0013-7227
VL - 148
SP - 21
EP - 26
JO - Endocrinology
JF - Endocrinology
IS - 1
ER -