Effects of nanoparticle coatings on the activity of oncolytic adenovirus-magnetic nanoparticle complexes

Nittaya Tresilwised, Pimolpan Pithayanukul, Per Sonne Holm, Ulrike Schillinger, Christian Plank, Olga Mykhaylyk

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Limitations to adenovirus infectivity can be overcome by association with magnetic nanoparticles and enforced infection by magnetic field influence. Here we examined three core-shell-type iron oxide magnetic nanoparticles differing in their surface coatings, particle sizes and magnetic properties for their ability to enhance the oncolytic potency of adenovirus Ad520 and to stabilize it against the inhibitory effects of serum or a neutralizing antibody. It was found that the physicochemical properties of magnetic nanoparticles are critical determinants of the properties which govern the oncolytic productivities of their complexes with Ad520. Although high serum concentration during infection or a neutralizing antibody had strong inhibitory influence on the uptake or oncolytic productivity of the naked virus, one particle type was identified which conferred high protection against both inhibitory factors while enhancing the oncolytic productivity of the internalized virus. This particle type equipped with a silica coating and adsorbed polyethylenimine, displaying a high magnetic moment and high saturation magnetization, mediated a 50% reduction of tumor growth rate versus control upon intratumoral injection of its complex with Ad520 and magnetic field influence, whereas Ad520 alone was inefficient. The correlations between physical properties of the magnetic particles or virus complexes and oncolytic potency are described herein.

Original languageEnglish
Pages (from-to)256-269
Number of pages14
JournalBiomaterials
Volume33
Issue number1
DOIs
StatePublished - Jan 2012

Keywords

  • Magnetic nanoparticles
  • Multidrug-resistant cancer cells
  • Oncolytic activity
  • Oncolytic adenovirus
  • Surface coating

Fingerprint

Dive into the research topics of 'Effects of nanoparticle coatings on the activity of oncolytic adenovirus-magnetic nanoparticle complexes'. Together they form a unique fingerprint.

Cite this