Effects of interfering and influencing factors on the analyses of p105 (c-erbB-2/HER-2) oncoprotein fragment in serum

Stephan Mielke, Harald Meden, Titus Raab, Wolfgang Wuttke, Walther Kuhn

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Background : The 105kDa extracellular domain of cerbB-2 encoded protein p185 is detectable in serum. Elevated p105 serum levels were found in patients with breast and ovarian cancer and in pregnancy. The effects of interfering and influencing factors on the measurements are still unclear. Material and methods: We used an ELISA based on the monoclonal capture antibody OD-3 and investigated possible interfering factors, like changing transporting and storing conditions of blood and serum samples. In order to evaluate a normal range of p105 serum values we examined 71 healthy women. Results: In our tests we found no influence on the reproducibility of results by changing transporting and storing conditions. We could not find any dependency of pl05 serum values on the menstrual cycle or the age of controls. Postmenopausal women showed significantly higher serum values than premenopausal women (p=0.0127). Conclusions: Clinical interpretation of female p105 serum values requires comparison with normal ranges when considering the menopause as an influencing factor.

Original languageEnglish
Pages (from-to)3125-3127
Number of pages3
JournalAnticancer Research
Volume17
Issue number4 B
StatePublished - Jul 1997
Externally publishedYes

Keywords

  • Cycle menopause
  • Menstrual
  • Normal
  • Range
  • Serum
  • p105

Fingerprint

Dive into the research topics of 'Effects of interfering and influencing factors on the analyses of p105 (c-erbB-2/HER-2) oncoprotein fragment in serum'. Together they form a unique fingerprint.

Cite this