TY - JOUR
T1 - Effects of increase in fish oil intake on intestinal eicosanoids and inflammation in a mouse model of colitis
AU - Bosco, Nabil
AU - Brahmbhatt, Viral
AU - Oliveira, Manuel
AU - Martin, Francois Pierre
AU - Lichti, Pia
AU - Raymond, Frederic
AU - Mansourian, Robert
AU - Metairon, Sylviane
AU - Pace-Asciak, Cecil
AU - Bastic Schmid, Viktoria
AU - Rezzi, Serge
AU - Haller, Dirk
AU - Benyacoub, Jalil
N1 - Funding Information:
This work was supported by Nestec SA. We thank immunology laboratory members for stimulating discussions and comments in the course of this work as well as NRC animal care team.
PY - 2013
Y1 - 2013
N2 - Background: Inflammatory bowel diseases (IBD) are chronic intestinal inflammatory diseases affecting about 1% of western populations. New eating behaviors might contribute to the global emergence of IBD. Although the immunoregulatory effects of omega-3 fatty acids have been well characterized in vitro, their role in IBD is controversial. Methods. The aim of this study was to assess the impact of increased fish oil intake on colonic gene expression, eicosanoid metabolism and development of colitis in a mouse model of IBD. Rag-2 deficient mice were fed fish oil (FO) enriched in omega-3 fatty acids i.e. EPA and DHA or control diet for 4 weeks before colitis induction by adoptive transfer of naïve T cells and maintained in the same diet for 4 additional weeks. Onset of colitis was monitored by colonoscopy and further confirmed by immunological examinations. Whole genome expression profiling was made and eicosanoids were measured by HPLC-MS/MS in colonic samples. Results: A significant reduction of colonic proinflammatory eicosanoids in FO fed mice compared to control was observed. However, neither alteration of colonic gene expression signature nor reduction in IBD scores was observed under FO diet. Conclusion: Thus, increased intake of dietary FO did not prevent experimental colitis.
AB - Background: Inflammatory bowel diseases (IBD) are chronic intestinal inflammatory diseases affecting about 1% of western populations. New eating behaviors might contribute to the global emergence of IBD. Although the immunoregulatory effects of omega-3 fatty acids have been well characterized in vitro, their role in IBD is controversial. Methods. The aim of this study was to assess the impact of increased fish oil intake on colonic gene expression, eicosanoid metabolism and development of colitis in a mouse model of IBD. Rag-2 deficient mice were fed fish oil (FO) enriched in omega-3 fatty acids i.e. EPA and DHA or control diet for 4 weeks before colitis induction by adoptive transfer of naïve T cells and maintained in the same diet for 4 additional weeks. Onset of colitis was monitored by colonoscopy and further confirmed by immunological examinations. Whole genome expression profiling was made and eicosanoids were measured by HPLC-MS/MS in colonic samples. Results: A significant reduction of colonic proinflammatory eicosanoids in FO fed mice compared to control was observed. However, neither alteration of colonic gene expression signature nor reduction in IBD scores was observed under FO diet. Conclusion: Thus, increased intake of dietary FO did not prevent experimental colitis.
KW - Docosahexaenoic acid
KW - Eicosanoids
KW - Eicosapentaenoic acid
KW - Inflammation
KW - Inflammatory bowel disease
KW - Omega-3 fatty acids
UR - http://www.scopus.com/inward/record.url?scp=84878358567&partnerID=8YFLogxK
U2 - 10.1186/1476-511X-12-81
DO - 10.1186/1476-511X-12-81
M3 - Article
C2 - 23725086
AN - SCOPUS:84878358567
SN - 1476-511X
VL - 12
JO - Lipids in Health and Disease
JF - Lipids in Health and Disease
IS - 1
M1 - 81
ER -
