Effect of zinc deficiency on the mRNA expression pattern in liver and jejunum of adult rats: Monitoring gene expression using cDNA microarrays combined with real-time RT-PCR

Michael W. Pfaffl; B. Gerstmayer; A. Bosio; Wilhelm Windisch

doi:10.1016/j.jnutbio.2003.08.007

Effect of zinc deficiency on the mRNA expression pattern in liver and jejunum of adult rats: Monitoring gene expression using cDNA microarrays combined with real-time RT-PCR

Michael W. Pfaffl, B. Gerstmayer, A. Bosio, Wilhelm Windisch

Medical Molecular Research Cologne

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59 Scopus citations

Abstract

In the study presented here, the effect of zinc deficiency on mRNA expression levels in liver and jejunum of adult rats was analyzed. Feed intake was restricted to 8 g/day. The semi-synthetic diet was fortified with pure phytate and contained either 2 μg Zn/g (Zn deficiency, n = 6) or 58 μg Zn/g (control, n = 7). After 29 days of Zn depletion feeding, entire jejunum and liver were retrieved and total RNA was extracted. Tissue specific expression pattern were screened and quantified by microarray analysis and verified individually via real-time RT-PCR. A relative quantification was performed with the newly developed Relative Expression Software Tool

Original language	English
Pages (from-to)	691-702
Number of pages	12
Journal	Journal of Nutritional Biochemistry
Volume	14
Issue number	12
DOIs	https://doi.org/10.1016/j.jnutbio.2003.08.007
State	Published - Dec 2003

Keywords

Adult rats
Gene expression pattern
Metallothionein
Real-time RT-PCR
Relative expression
Zinc deficiency
cDNA microarray

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10.1016/j.jnutbio.2003.08.007

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title = "Effect of zinc deficiency on the mRNA expression pattern in liver and jejunum of adult rats: Monitoring gene expression using cDNA microarrays combined with real-time RT-PCR",

abstract = "In the study presented here, the effect of zinc deficiency on mRNA expression levels in liver and jejunum of adult rats was analyzed. Feed intake was restricted to 8 g/day. The semi-synthetic diet was fortified with pure phytate and contained either 2 μg Zn/g (Zn deficiency, n = 6) or 58 μg Zn/g (control, n = 7). After 29 days of Zn depletion feeding, entire jejunum and liver were retrieved and total RNA was extracted. Tissue specific expression pattern were screened and quantified by microarray analysis and verified individually via real-time RT-PCR. A relative quantification was performed with the newly developed Relative Expression Software Tool",

keywords = "Adult rats, Gene expression pattern, Metallothionein, Real-time RT-PCR, Relative expression, Zinc deficiency, cDNA microarray",

author = "Pfaffl, {Michael W.} and B. Gerstmayer and A. Bosio and Wilhelm Windisch",

note = "Funding Information: The authors wish to thank the Deutsche Forschungsgemeinschaft (DFG) for supporting this study by a grant. In addition we thank G. Grosshauser (memorec) and D. Tetzlaff (Institute of Physiology) for excellent technical assistance. ",

year = "2003",

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doi = "10.1016/j.jnutbio.2003.08.007",

language = "English",

volume = "14",

pages = "691--702",

journal = "Journal of Nutritional Biochemistry",

issn = "0955-2863",

publisher = "Elsevier Inc.",

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Effect of zinc deficiency on the mRNA expression pattern in liver and jejunum of adult rats: Monitoring gene expression using cDNA microarrays combined with real-time RT-PCR. / Pfaffl, Michael W.; Gerstmayer, B.; Bosio, A. et al.
In: Journal of Nutritional Biochemistry, Vol. 14, No. 12, 12.2003, p. 691-702.

Research output: Contribution to journal › Article › peer-review

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T1 - Effect of zinc deficiency on the mRNA expression pattern in liver and jejunum of adult rats

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AU - Pfaffl, Michael W.

AU - Gerstmayer, B.

AU - Bosio, A.

AU - Windisch, Wilhelm

N1 - Funding Information: The authors wish to thank the Deutsche Forschungsgemeinschaft (DFG) for supporting this study by a grant. In addition we thank G. Grosshauser (memorec) and D. Tetzlaff (Institute of Physiology) for excellent technical assistance.

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KW - Gene expression pattern

KW - Metallothionein

KW - Real-time RT-PCR

KW - Relative expression

KW - Zinc deficiency

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Effect of zinc deficiency on the mRNA expression pattern in liver and jejunum of adult rats: Monitoring gene expression using cDNA microarrays combined with real-time RT-PCR

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Keywords

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