Effect of vasoactive intestinal peptide, peptide histidine isoleucine and growth hormone-releasing factor-40 on bombesin-like immunoreactivity, somatostatin and gastrin release from the perfused rat stomach

V. Schusdziarra; R. Schmid; H. Bender; M. Schusdziarra; J. Rivier; W. Vale; M. Classen

doi:10.1016/0196-9781(86)90072-0

Effect of vasoactive intestinal peptide, peptide histidine isoleucine and growth hormone-releasing factor-40 on bombesin-like immunoreactivity, somatostatin and gastrin release from the perfused rat stomach

V. Schusdziarra, R. Schmid, H. Bender, M. Schusdziarra, J. Rivier, W. Vale, M. Classen

Department of Preventive Sports Medicine and Sports Cardiology

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11 Scopus citations

Abstract

Bombesin-like immunoreactivity (BLI) has been demonstrated in neurons of the gastrointestinal tract and gastric BLI secretion can be demonstrated in response to the classical neurotransmitter acetylcholine. Since structurally related peptides VIP, PHI and GRF have to be considered as peptidergic neurotransmitters it was of interest to determine their effect on gastric BLI secretion. Additionally, somatostatin (SLI) and gastrin secretion was examined. The isolated stomach of overnight fasted rats was perfused with Krebs-Ringer buffer via the celiac artery and the effluent was collected via the portal vein. The gastric lumen was perfused with isotonic saline at pH7 or pH2. All four peptides were tested at a dose of 10^-11 M and 10^-8 M at both pH levels and in addition the effect of VIP and PHI was examined at 10^-14 M and 10^-12 M during luminal pH2. At luminal pH7 VIP and PHI stimulated SLI release at 10^-8 M but had no effect on BLI or gastrin secretion. rGRF and hpGRF were both ineffective on SLI and gastrin release while rGRF inhibited and hpGRF stimulated BLI secretion. This effect was not dose related. At luminal pH2 all four peptides stimulated BLI secretion. Stimulation by PHI was already observed at a dose of 10^-14 M while VIP elicited a stimulatory effect at 10^-12 M. PHI at the two lowest concentrations of 10^-14 and 10^-12 M elicited a stimualtion of SLI and gastrin release while the same doses of VIP and the higher doses of all four peptides had no effect on SLI and gastrin secretion at an acidic intraluminal pH. The present data demonstrate that at a low intragastric pH BLI secretion is stimulated predominantly by putative peptidergic neurotransmitters such as PHI and VIP and possibly GRF in contrast to the previously reported cholinergic stimulation at neutral pH. The effect of low dose PHI on gastrin secretion suggests that this peptide might participate in the previously observed vagally-induced gastrin secretion during low intragastric pH. On the other hand, VIP and PHI might contribute to postprandial stimulation of somatostatin release during meal-induced increase of the intragastric pH.

Original language	English
Pages (from-to)	127-133
Number of pages	7
Journal	Peptides
Volume	7
Issue number	1
DOIs	https://doi.org/10.1016/0196-9781(86)90072-0
State	Published - 1986

Keywords

Bombesin
Gastrin
Growth hormone-releasing factor
Peptide histidine isoleucine
Peptidergic neurotransmitter
Somatostatin
Stomach
Vasoactive intestinal peptide

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10.1016/0196-9781(86)90072-0

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Schusdziarra, V., Schmid, R., Bender, H., Schusdziarra, M., Rivier, J., Vale, W., & Classen, M. (1986). Effect of vasoactive intestinal peptide, peptide histidine isoleucine and growth hormone-releasing factor-40 on bombesin-like immunoreactivity, somatostatin and gastrin release from the perfused rat stomach. Peptides, 7(1), 127-133. https://doi.org/10.1016/0196-9781(86)90072-0

@article{062f2689750041b985b88d265227e099,

title = "Effect of vasoactive intestinal peptide, peptide histidine isoleucine and growth hormone-releasing factor-40 on bombesin-like immunoreactivity, somatostatin and gastrin release from the perfused rat stomach",

abstract = "Bombesin-like immunoreactivity (BLI) has been demonstrated in neurons of the gastrointestinal tract and gastric BLI secretion can be demonstrated in response to the classical neurotransmitter acetylcholine. Since structurally related peptides VIP, PHI and GRF have to be considered as peptidergic neurotransmitters it was of interest to determine their effect on gastric BLI secretion. Additionally, somatostatin (SLI) and gastrin secretion was examined. The isolated stomach of overnight fasted rats was perfused with Krebs-Ringer buffer via the celiac artery and the effluent was collected via the portal vein. The gastric lumen was perfused with isotonic saline at pH7 or pH2. All four peptides were tested at a dose of 10-11 M and 10-8 M at both pH levels and in addition the effect of VIP and PHI was examined at 10-14 M and 10-12 M during luminal pH2. At luminal pH7 VIP and PHI stimulated SLI release at 10-8 M but had no effect on BLI or gastrin secretion. rGRF and hpGRF were both ineffective on SLI and gastrin release while rGRF inhibited and hpGRF stimulated BLI secretion. This effect was not dose related. At luminal pH2 all four peptides stimulated BLI secretion. Stimulation by PHI was already observed at a dose of 10-14 M while VIP elicited a stimulatory effect at 10-12 M. PHI at the two lowest concentrations of 10-14 and 10-12 M elicited a stimualtion of SLI and gastrin release while the same doses of VIP and the higher doses of all four peptides had no effect on SLI and gastrin secretion at an acidic intraluminal pH. The present data demonstrate that at a low intragastric pH BLI secretion is stimulated predominantly by putative peptidergic neurotransmitters such as PHI and VIP and possibly GRF in contrast to the previously reported cholinergic stimulation at neutral pH. The effect of low dose PHI on gastrin secretion suggests that this peptide might participate in the previously observed vagally-induced gastrin secretion during low intragastric pH. On the other hand, VIP and PHI might contribute to postprandial stimulation of somatostatin release during meal-induced increase of the intragastric pH.",

keywords = "Bombesin, Gastrin, Growth hormone-releasing factor, Peptide histidine isoleucine, Peptidergic neurotransmitter, Somatostatin, Stomach, Vasoactive intestinal peptide",

author = "V. Schusdziarra and R. Schmid and H. Bender and M. Schusdziarra and J. Rivier and W. Vale and M. Classen",

note = "Funding Information: The authors would like to thank C. Bullmann and H. Czelhan for expert technical assistance. The work was supported by Deutsche Forschungsgemeinschaft SFB 87 G6.",

year = "1986",

doi = "10.1016/0196-9781(86)90072-0",

language = "English",

volume = "7",

pages = "127--133",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "1",

}

Schusdziarra, V, Schmid, R, Bender, H, Schusdziarra, M, Rivier, J, Vale, W & Classen, M 1986, 'Effect of vasoactive intestinal peptide, peptide histidine isoleucine and growth hormone-releasing factor-40 on bombesin-like immunoreactivity, somatostatin and gastrin release from the perfused rat stomach', Peptides, vol. 7, no. 1, pp. 127-133. https://doi.org/10.1016/0196-9781(86)90072-0

Effect of vasoactive intestinal peptide, peptide histidine isoleucine and growth hormone-releasing factor-40 on bombesin-like immunoreactivity, somatostatin and gastrin release from the perfused rat stomach. / Schusdziarra, V.; Schmid, R.; Bender, H. et al.
In: Peptides, Vol. 7, No. 1, 1986, p. 127-133.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Effect of vasoactive intestinal peptide, peptide histidine isoleucine and growth hormone-releasing factor-40 on bombesin-like immunoreactivity, somatostatin and gastrin release from the perfused rat stomach

AU - Schusdziarra, V.

AU - Schmid, R.

AU - Bender, H.

AU - Schusdziarra, M.

AU - Rivier, J.

AU - Vale, W.

AU - Classen, M.

N1 - Funding Information: The authors would like to thank C. Bullmann and H. Czelhan for expert technical assistance. The work was supported by Deutsche Forschungsgemeinschaft SFB 87 G6.

PY - 1986

Y1 - 1986

N2 - Bombesin-like immunoreactivity (BLI) has been demonstrated in neurons of the gastrointestinal tract and gastric BLI secretion can be demonstrated in response to the classical neurotransmitter acetylcholine. Since structurally related peptides VIP, PHI and GRF have to be considered as peptidergic neurotransmitters it was of interest to determine their effect on gastric BLI secretion. Additionally, somatostatin (SLI) and gastrin secretion was examined. The isolated stomach of overnight fasted rats was perfused with Krebs-Ringer buffer via the celiac artery and the effluent was collected via the portal vein. The gastric lumen was perfused with isotonic saline at pH7 or pH2. All four peptides were tested at a dose of 10-11 M and 10-8 M at both pH levels and in addition the effect of VIP and PHI was examined at 10-14 M and 10-12 M during luminal pH2. At luminal pH7 VIP and PHI stimulated SLI release at 10-8 M but had no effect on BLI or gastrin secretion. rGRF and hpGRF were both ineffective on SLI and gastrin release while rGRF inhibited and hpGRF stimulated BLI secretion. This effect was not dose related. At luminal pH2 all four peptides stimulated BLI secretion. Stimulation by PHI was already observed at a dose of 10-14 M while VIP elicited a stimulatory effect at 10-12 M. PHI at the two lowest concentrations of 10-14 and 10-12 M elicited a stimualtion of SLI and gastrin release while the same doses of VIP and the higher doses of all four peptides had no effect on SLI and gastrin secretion at an acidic intraluminal pH. The present data demonstrate that at a low intragastric pH BLI secretion is stimulated predominantly by putative peptidergic neurotransmitters such as PHI and VIP and possibly GRF in contrast to the previously reported cholinergic stimulation at neutral pH. The effect of low dose PHI on gastrin secretion suggests that this peptide might participate in the previously observed vagally-induced gastrin secretion during low intragastric pH. On the other hand, VIP and PHI might contribute to postprandial stimulation of somatostatin release during meal-induced increase of the intragastric pH.

AB - Bombesin-like immunoreactivity (BLI) has been demonstrated in neurons of the gastrointestinal tract and gastric BLI secretion can be demonstrated in response to the classical neurotransmitter acetylcholine. Since structurally related peptides VIP, PHI and GRF have to be considered as peptidergic neurotransmitters it was of interest to determine their effect on gastric BLI secretion. Additionally, somatostatin (SLI) and gastrin secretion was examined. The isolated stomach of overnight fasted rats was perfused with Krebs-Ringer buffer via the celiac artery and the effluent was collected via the portal vein. The gastric lumen was perfused with isotonic saline at pH7 or pH2. All four peptides were tested at a dose of 10-11 M and 10-8 M at both pH levels and in addition the effect of VIP and PHI was examined at 10-14 M and 10-12 M during luminal pH2. At luminal pH7 VIP and PHI stimulated SLI release at 10-8 M but had no effect on BLI or gastrin secretion. rGRF and hpGRF were both ineffective on SLI and gastrin release while rGRF inhibited and hpGRF stimulated BLI secretion. This effect was not dose related. At luminal pH2 all four peptides stimulated BLI secretion. Stimulation by PHI was already observed at a dose of 10-14 M while VIP elicited a stimulatory effect at 10-12 M. PHI at the two lowest concentrations of 10-14 and 10-12 M elicited a stimualtion of SLI and gastrin release while the same doses of VIP and the higher doses of all four peptides had no effect on SLI and gastrin secretion at an acidic intraluminal pH. The present data demonstrate that at a low intragastric pH BLI secretion is stimulated predominantly by putative peptidergic neurotransmitters such as PHI and VIP and possibly GRF in contrast to the previously reported cholinergic stimulation at neutral pH. The effect of low dose PHI on gastrin secretion suggests that this peptide might participate in the previously observed vagally-induced gastrin secretion during low intragastric pH. On the other hand, VIP and PHI might contribute to postprandial stimulation of somatostatin release during meal-induced increase of the intragastric pH.

KW - Bombesin

KW - Gastrin

KW - Growth hormone-releasing factor

KW - Peptide histidine isoleucine

KW - Peptidergic neurotransmitter

KW - Somatostatin

KW - Stomach

KW - Vasoactive intestinal peptide

UR - http://www.scopus.com/inward/record.url?scp=0022448157&partnerID=8YFLogxK

U2 - 10.1016/0196-9781(86)90072-0

DO - 10.1016/0196-9781(86)90072-0

M3 - Article

C2 - 2872659

AN - SCOPUS:0022448157

SN - 0196-9781

VL - 7

SP - 127

EP - 133

JO - Peptides

JF - Peptides

IS - 1

ER -

Effect of vasoactive intestinal peptide, peptide histidine isoleucine and growth hormone-releasing factor-40 on bombesin-like immunoreactivity, somatostatin and gastrin release from the perfused rat stomach

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Keywords

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