Effect of hypoxia preconditioned secretomes on lymphangiogenic and angiogenic sprouting: An in vitro analysis

Philipp Moog, Rahmin Schams, Alexander Schneidinger, Arndt F. Schilling, Hans Günther Machens, Ektoras Hadjipanayi, Ulf Dornseifer

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Hypoxia Preconditioned Plasma (HPP) and Serum (HPS) are two blood-derived autologous growth factor compositions that are being clinically employed as tools for promoting tissue regeneration, and have been extensively examined for their angiogenic activity. As yet, their ability to stimulate/support lymphangiogenesis remains unknown, although this is an important but often-neglected process in wound healing and tissue repair. Here we set out to characterize the potential of hypoxia preconditioned secretomes as promoters of angiogenic and lymphangiogenic sprouting in vitro. We first analysed HPP/HPS in terms of pro- (VEGF-C) and anti- (TSP-1, PF-4) angiogenic/lymphangiogenic growth factor concentration, before testing their ability to stimulate microvessel sprouting in the mouse aortic ring assay and lymphatic sprouting in the thoracic duct ring assay. The origin of lymphatic structures was validated with lymph-specific immunohistochemical staining (Anti-LYVE-1) and lymphatic vessel-associated protein (polydom) quantification in culture supernatants. HPP/HPS induced greater angiogenic and lymphatic sprouting compared to non-hypoxia preconditioned samples (normal plasma/serum), a response that was compatible with their higher VEGF-C concentration. These findings demonstrate that hypoxia preconditioned blood-derived secretomes have the ability to not only support sprouting angiogenesis, but also lymphangiogenesis, which underlines their multimodal regenerative potential.

Original languageEnglish
Article number365
Issue number9
StatePublished - Sep 2020
Externally publishedYes


  • Adipose-derived cell supension
  • Adipose-derived stem cells
  • Angiogenesis
  • Blood-derived therapy
  • Hypoxia
  • Hypoxia preconditioned plasma
  • Hypoxia preconditioned serum
  • Lymphangiogenesis
  • Lymphatic regeneration
  • Peripheral blood cells


Dive into the research topics of 'Effect of hypoxia preconditioned secretomes on lymphangiogenic and angiogenic sprouting: An in vitro analysis'. Together they form a unique fingerprint.

Cite this