Abstract
Background: Plasmacytoid dendritic cells (PDCs) infiltrating solid tumor tissues and draining lymph nodes of head and neck squamous cell carcinoma (HNSCC) show an impaired immune response. Immunosuppressive cytokines secreted by HNSCC have been recognized to account for reduced interferon-α (IFN-α) production, whereas the influence on PDC migration has not yet been investigated. Materials and Methods: PDCs were isolated from human peripheral blood by magnetic bead separation. Cellular functions and characteristics were analyzed using flow cytometry, migration assays and ELISA techniques. Results: We show that HNSCC and CpG oligonucleotides controversially influence the migration and IFN-α production of PDCs. Furthermore we demonstrate that HNSCC induced migration towards stromal cell-derived factor-1 (SDF-1) and macrophage inflammatory protein 1 β (MIP-1β) is not dependent upon the surface density of chemokine (CXC motif) receptor 4 (CXCR4) and chemokine (C-C motif) receptor 5 (CCR5). Conclusion: We propose that HNSCC triggered PDC migration is most likely linked to a multilevel process strongly modulated by tumor microenvironmental influences.
| Original language | English |
|---|---|
| Pages (from-to) | 3019-3025 |
| Number of pages | 7 |
| Journal | Anticancer Research |
| Volume | 29 |
| Issue number | 8 |
| State | Published - Aug 2009 |
| Externally published | Yes |
Keywords
- CpG
- HNSCC
- IFN-α
- Migration
- Plasmacytoid dendritic cell
