Effect of 15-hydroxyeicosatetraenoic acid (15-HETE) on anti-immunglobulin E- and calcium ionophore-induced histamine release from human leukocytes: Comparison with the effects of eicosatetraenoic acid and nordihydroguaretic acid

15-Hydroxyeicosatetraenoic acid (15-HETE) was prepared by soybean lipoxygenase-mediated oxygenation of arachidonic acid to 15-hydroperoxyeicosatetraenoic acid (15-HPETE) and subsequent reduction by NaBH₄. 15-HETE was identified, purified and proved as biologically active by thin layer chromatography, high pressure liquid chromatography, gas chromatography/mass spectrometry and biological experiments on horse thrombocytes and rabbit peritoneal leukocytes. 15-HETE (0.1-40 μM) was added to peripheral leukocytes of 48 human donors (33 atopies, 15 nonatopics) which were challenged with rabbit anti-human-IgE or calcium ionophore A 23187. Its effect was compared with the effects of nordihydroguaretic acid (NDGA) and eicosatetraynoic acid (ETYA). NDGA and ETYA markedly inhibited histamine release (17 μM: 74± 11 and 39 ± 14, 3%, respectively), whereas 15-HETE neither stimulated nor inhibited spontaneous anti-IgE- or calcium ionophore A 23187-induced histamine release.