TY - JOUR
T1 - Effect of 15-hydroxyeicosatetraenoic acid (15-HETE) on anti-immunglobulin E- and calcium ionophore-induced histamine release from human leukocytes
T2 - Comparison with the effects of eicosatetraenoic acid and nordihydroguaretic acid
AU - Dorsch, Walter
AU - Ring, Johannes
AU - Riepel, Herbert
PY - 1984
Y1 - 1984
N2 - 15-Hydroxyeicosatetraenoic acid (15-HETE) was prepared by soybean lipoxygenase-mediated oxygenation of arachidonic acid to 15-hydroperoxyeicosatetraenoic acid (15-HPETE) and subsequent reduction by NaBH4. 15-HETE was identified, purified and proved as biologically active by thin layer chromatography, high pressure liquid chromatography, gas chromatography/mass spectrometry and biological experiments on horse thrombocytes and rabbit peritoneal leukocytes. 15-HETE (0.1-40 μM) was added to peripheral leukocytes of 48 human donors (33 atopies, 15 nonatopics) which were challenged with rabbit anti-human-IgE or calcium ionophore A 23187. Its effect was compared with the effects of nordihydroguaretic acid (NDGA) and eicosatetraynoic acid (ETYA). NDGA and ETYA markedly inhibited histamine release (17 μM: 74± 11 and 39 ± 14, 3%, respectively), whereas 15-HETE neither stimulated nor inhibited spontaneous anti-IgE- or calcium ionophore A 23187-induced histamine release.
AB - 15-Hydroxyeicosatetraenoic acid (15-HETE) was prepared by soybean lipoxygenase-mediated oxygenation of arachidonic acid to 15-hydroperoxyeicosatetraenoic acid (15-HPETE) and subsequent reduction by NaBH4. 15-HETE was identified, purified and proved as biologically active by thin layer chromatography, high pressure liquid chromatography, gas chromatography/mass spectrometry and biological experiments on horse thrombocytes and rabbit peritoneal leukocytes. 15-HETE (0.1-40 μM) was added to peripheral leukocytes of 48 human donors (33 atopies, 15 nonatopics) which were challenged with rabbit anti-human-IgE or calcium ionophore A 23187. Its effect was compared with the effects of nordihydroguaretic acid (NDGA) and eicosatetraynoic acid (ETYA). NDGA and ETYA markedly inhibited histamine release (17 μM: 74± 11 and 39 ± 14, 3%, respectively), whereas 15-HETE neither stimulated nor inhibited spontaneous anti-IgE- or calcium ionophore A 23187-induced histamine release.
UR - http://www.scopus.com/inward/record.url?scp=0021346136&partnerID=8YFLogxK
U2 - 10.1159/000233481
DO - 10.1159/000233481
M3 - Article
C2 - 6199312
AN - SCOPUS:0021346136
SN - 1018-2438
VL - 73
SP - 274
EP - 279
JO - International Archives of Allergy and Immunology
JF - International Archives of Allergy and Immunology
IS - 3
ER -