TY - JOUR
T1 - Dynamics in multi-domain protein recognition of RNA
AU - Mackereth, Cameron D.
AU - Sattler, Michael
N1 - Funding Information:
We thank Janosch Hennig, Hyun-Seo Kang, Hamed Kooshapur and Thomas Kern for comments and critical reading of the manuscript. We apologize to those colleagues whose work we could not further discuss owing to space limitations. CDM thanks the European Molecular Biology Organization for short term funding. Research in the authors’ laboratories is supported by the Institut Européen de Chimie et Biologie , Institut National de la Santé et de la Recherche Médicale , Aquitaine Regional government , Helmholtz Association , the Deutsche Forschungsgemeinschaft (Cluster of excellence CiPSM) , and the FP7 Programme of the European Commission .
PY - 2012/6
Y1 - 2012/6
N2 - Protein-RNA interactions play essential roles in gene regulation and RNA metabolism. While high-resolution structures have revealed principles of RNA recognition by individual RNA binding domains (RBDs), the presence of multiple RBDs in many eukaryotic proteins suggests additional modes of RNA recognition by combination and cooperation of these interactions. Recent structures, together with biochemical and biophysical studies have revealed novel principles of RNA recognition by multi-domain proteins. These examples highlight an important role for dynamics in RNA recognition, with mechanisms including fly-casting and conformational selection, and advocate the use of solution techniques for their analysis.
AB - Protein-RNA interactions play essential roles in gene regulation and RNA metabolism. While high-resolution structures have revealed principles of RNA recognition by individual RNA binding domains (RBDs), the presence of multiple RBDs in many eukaryotic proteins suggests additional modes of RNA recognition by combination and cooperation of these interactions. Recent structures, together with biochemical and biophysical studies have revealed novel principles of RNA recognition by multi-domain proteins. These examples highlight an important role for dynamics in RNA recognition, with mechanisms including fly-casting and conformational selection, and advocate the use of solution techniques for their analysis.
UR - http://www.scopus.com/inward/record.url?scp=84862633452&partnerID=8YFLogxK
U2 - 10.1016/j.sbi.2012.03.013
DO - 10.1016/j.sbi.2012.03.013
M3 - Review article
C2 - 22516180
AN - SCOPUS:84862633452
SN - 0959-440X
VL - 22
SP - 287
EP - 296
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
IS - 3
ER -