Dynamic force sensing of filamin revealed in single-molecule experiments

Lorenz Rognoni, Johannes Stigler, Benjamin Pelz, Jari Ylänne, Matthias Rief

Research output: Contribution to journalArticlepeer-review

124 Scopus citations

Abstract

Mechanical forces are important signals for cell response and development, but detailed molecular mechanisms of force sensing are largely unexplored. The cytoskeletal protein filamin is a key connecting element between the cytoskeleton and transmembrane complexes such as integrins or the von Willebrand receptor glycoprotein Ib. Here, we show using single-molecule mechanical measurements that the recently reported Ig domain pair 20-21 of human filamin A acts as an autoinhibited force-activatable mechanosensor. We developed a mechanical single-molecule competition assay that allows online observation of binding events of target peptides in solution to the strained domain pair. We find that filamin force sensing is a highly dynamic process occurring in rapid equilibrium that increases the affinity to the target peptides by up to a factor of 17 between 2 and 5 pN. The equilibrium mechanism we find here can offer a general scheme for cellular force sensing.

Original languageEnglish
Pages (from-to)19679-19684
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number48
DOIs
StatePublished - 27 Nov 2012

Keywords

  • Mechanosensing
  • Optical tweezers

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