Dual-Modality Optical/PET Imaging of PARP1 in Glioblastoma

Giuseppe Carlucci; Brandon Carney; Christian Brand; Susanne Kossatz; Christopher P. Irwin; Sean D. Carlin; Edmund J. Keliher; Wolfgang Weber; Thomas Reiner

doi:10.1007/s11307-015-0858-0

Dual-Modality Optical/PET Imaging of PARP1 in Glioblastoma

Giuseppe Carlucci, Brandon Carney, Christian Brand, Susanne Kossatz, Christopher P. Irwin, Sean D. Carlin, Edmund J. Keliher, Wolfgang Weber, Thomas Reiner

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

Purpose: The current study presents [¹⁸F]PARPi-FL as a bimodal fluorescent/positron emission tomography (PET) agent for PARP1 imaging. Procedures: [¹⁸F]PARPi-FL was obtained by ¹⁹F/¹⁸F isotopic exchange and PET experiments, biodistribution studies, surface fluorescence imaging, and autoradiography carried out in a U87 MG glioblastoma mouse model. Results: [¹⁸F]PARPi-FL showed high tumor uptake in vivo and ex vivo in small xenografts (< 2 mm) with both PET and optical imaging technologies. Uptake of [¹⁸F]PARPi-FL in blocked U87 MG tumors was reduced by 84 % (0.12 ± 0.02 %injected dose/gram (%ID/g)), showing high specificity of the binding. PET imaging showed accumulation in the tumor (1 h p.i.), which was confirmed by ex vivo phosphor autoradiography. Conclusions: The fluorescent component of [¹⁸F]PARPi-FL enables cellular resolution optical imaging, while the radiolabeled component of [¹⁸F]PARPi-FL allows whole-body deep-tissue imaging of malignant growth.

Original language	English
Pages (from-to)	848-855
Number of pages	8
Journal	Molecular Imaging and Biology
Volume	17
Issue number	6
DOIs	https://doi.org/10.1007/s11307-015-0858-0
State	Published - 1 Dec 2015
Externally published	Yes

Keywords

Fluorescence
Glioblastoma
Imaging
Multimodality
PARP1
PET
U87 MG

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Dual-Modality Optical/PET Imaging of PARP1 in Glioblastoma",

abstract = "Purpose: The current study presents [18F]PARPi-FL as a bimodal fluorescent/positron emission tomography (PET) agent for PARP1 imaging. Procedures: [18F]PARPi-FL was obtained by 19F/18F isotopic exchange and PET experiments, biodistribution studies, surface fluorescence imaging, and autoradiography carried out in a U87 MG glioblastoma mouse model. Results: [18F]PARPi-FL showed high tumor uptake in vivo and ex vivo in small xenografts (< 2 mm) with both PET and optical imaging technologies. Uptake of [18F]PARPi-FL in blocked U87 MG tumors was reduced by 84 % (0.12 ± 0.02 %injected dose/gram (%ID/g)), showing high specificity of the binding. PET imaging showed accumulation in the tumor (1 h p.i.), which was confirmed by ex vivo phosphor autoradiography. Conclusions: The fluorescent component of [18F]PARPi-FL enables cellular resolution optical imaging, while the radiolabeled component of [18F]PARPi-FL allows whole-body deep-tissue imaging of malignant growth.",

keywords = "Fluorescence, Glioblastoma, Imaging, Multimodality, PARP1, PET, U87 MG",

author = "Giuseppe Carlucci and Brandon Carney and Christian Brand and Susanne Kossatz and Irwin, {Christopher P.} and Carlin, {Sean D.} and Keliher, {Edmund J.} and Wolfgang Weber and Thomas Reiner",

note = "Publisher Copyright: {\textcopyright} 2015, World Molecular Imaging Society.",

year = "2015",

month = dec,

day = "1",

doi = "10.1007/s11307-015-0858-0",

language = "English",

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TY - JOUR

T1 - Dual-Modality Optical/PET Imaging of PARP1 in Glioblastoma

AU - Carlucci, Giuseppe

AU - Carney, Brandon

AU - Brand, Christian

AU - Kossatz, Susanne

AU - Irwin, Christopher P.

AU - Carlin, Sean D.

AU - Keliher, Edmund J.

AU - Weber, Wolfgang

AU - Reiner, Thomas

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Purpose: The current study presents [18F]PARPi-FL as a bimodal fluorescent/positron emission tomography (PET) agent for PARP1 imaging. Procedures: [18F]PARPi-FL was obtained by 19F/18F isotopic exchange and PET experiments, biodistribution studies, surface fluorescence imaging, and autoradiography carried out in a U87 MG glioblastoma mouse model. Results: [18F]PARPi-FL showed high tumor uptake in vivo and ex vivo in small xenografts (< 2 mm) with both PET and optical imaging technologies. Uptake of [18F]PARPi-FL in blocked U87 MG tumors was reduced by 84 % (0.12 ± 0.02 %injected dose/gram (%ID/g)), showing high specificity of the binding. PET imaging showed accumulation in the tumor (1 h p.i.), which was confirmed by ex vivo phosphor autoradiography. Conclusions: The fluorescent component of [18F]PARPi-FL enables cellular resolution optical imaging, while the radiolabeled component of [18F]PARPi-FL allows whole-body deep-tissue imaging of malignant growth.

AB - Purpose: The current study presents [18F]PARPi-FL as a bimodal fluorescent/positron emission tomography (PET) agent for PARP1 imaging. Procedures: [18F]PARPi-FL was obtained by 19F/18F isotopic exchange and PET experiments, biodistribution studies, surface fluorescence imaging, and autoradiography carried out in a U87 MG glioblastoma mouse model. Results: [18F]PARPi-FL showed high tumor uptake in vivo and ex vivo in small xenografts (< 2 mm) with both PET and optical imaging technologies. Uptake of [18F]PARPi-FL in blocked U87 MG tumors was reduced by 84 % (0.12 ± 0.02 %injected dose/gram (%ID/g)), showing high specificity of the binding. PET imaging showed accumulation in the tumor (1 h p.i.), which was confirmed by ex vivo phosphor autoradiography. Conclusions: The fluorescent component of [18F]PARPi-FL enables cellular resolution optical imaging, while the radiolabeled component of [18F]PARPi-FL allows whole-body deep-tissue imaging of malignant growth.

KW - Fluorescence

KW - Glioblastoma

KW - Imaging

KW - Multimodality

KW - PARP1

KW - PET

KW - U87 MG

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ER -

Dual-Modality Optical/PET Imaging of PARP1 in Glioblastoma

Abstract

Keywords

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