TY - JOUR
T1 - Dual action of isoflurane on the γ-aminobutyric acid (GABA)-mediated currents through recombinant α1β2γ(2L)-GABA(A)-receptor channels
AU - Neumahr, Susanne
AU - Hapfelmeier, Gerhard
AU - Scheller, Michaela
AU - Schneck, Hajo
AU - Franke, Christian
AU - Kochs, Eberhard
PY - 2000
Y1 - 2000
N2 - Isoflurane (ISO) increased the agonist-induced chloride flux through the γ-aminobutyric acid A receptor (GABA(A)R). This may reflect an anesthetic- induced increase in the apparent agonist affinity. A dual effect of anesthetics was postulated for both the nicotinic acetylcholine receptor (nAChR) and the GABA(A)R. We tested the hypothesis that, in addition to a blocking effect, ISO increases γ-aminobutyric acid (GABA)-gated currents through recombinant GABA(A)R channels. HEK293 cells were transfected with rat cDNA for α1,β2,γ(2L) subunits. Currents elicited by 1 mM or 0.01 mM GABA, respectively, alone, or with increasing concentrations of ISO, were recorded by using standard patch clamp techniques. ISO reduced the peak current elicited by 1 mM GABA. Currents induced by 0.01 mM GABA were potentiated by small ISO (twofold at 0.5 mM ISO) and inhibited by larger concentrations. Withdrawal of ISO and GABA induced rebound currents, suggesting an open-channel block by ISO. These currents increased with increasing concentrations of ISO. At large concentrations of ISO, the inhibitory effect predominated and was caused by, at least partly, an open- channel block. At small concentrations of ISO, potentiation of the GABA-gated currents was more prominent. This dual action of ISO indicates different binding sites at the GABA(A)R. The balance between potentiation and block depends on the concentrations of both ISO and GABA.
AB - Isoflurane (ISO) increased the agonist-induced chloride flux through the γ-aminobutyric acid A receptor (GABA(A)R). This may reflect an anesthetic- induced increase in the apparent agonist affinity. A dual effect of anesthetics was postulated for both the nicotinic acetylcholine receptor (nAChR) and the GABA(A)R. We tested the hypothesis that, in addition to a blocking effect, ISO increases γ-aminobutyric acid (GABA)-gated currents through recombinant GABA(A)R channels. HEK293 cells were transfected with rat cDNA for α1,β2,γ(2L) subunits. Currents elicited by 1 mM or 0.01 mM GABA, respectively, alone, or with increasing concentrations of ISO, were recorded by using standard patch clamp techniques. ISO reduced the peak current elicited by 1 mM GABA. Currents induced by 0.01 mM GABA were potentiated by small ISO (twofold at 0.5 mM ISO) and inhibited by larger concentrations. Withdrawal of ISO and GABA induced rebound currents, suggesting an open-channel block by ISO. These currents increased with increasing concentrations of ISO. At large concentrations of ISO, the inhibitory effect predominated and was caused by, at least partly, an open- channel block. At small concentrations of ISO, potentiation of the GABA-gated currents was more prominent. This dual action of ISO indicates different binding sites at the GABA(A)R. The balance between potentiation and block depends on the concentrations of both ISO and GABA.
UR - http://www.scopus.com/inward/record.url?scp=0034020263&partnerID=8YFLogxK
U2 - 10.1097/00000539-200005000-00035
DO - 10.1097/00000539-200005000-00035
M3 - Article
C2 - 10781477
AN - SCOPUS:0034020263
SN - 0003-2999
VL - 90
SP - 1184
EP - 1190
JO - Anesthesia and Analgesia
JF - Anesthesia and Analgesia
IS - 5
ER -