Doxycycline inducible melanogenic vaccinia virus as theranostic anti-cancer agent

Lorenz Kirscher, Xosé Luis Deán-Ben, Miriam Scadeng, Angelika Zaremba, Qian Zhang, Christina Kober, Thomas Felix Fehm, Daniel Razansky, Vasilis Ntziachristos, Jochen Stritzker, Aladar A. Szalay

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


We reported earlier the diagnostic potential of a melanogenic vaccinia virus based system in magnetic resonance (MRI) and optoacoustic deep tissue imaging (MSOT). Since melanin overproduction lead to attenuated virus replication, we constructed a novel recombinant vaccinia virus strain (rVACV), GLV-1h462, which expressed the key enzyme of melanogenesis (tyrosinase) under the control of an inducible promoter-system. In this study melanin production was detected after exogenous addition of doxycycline in two different tumor xenograft mouse models. Furthermore, it was confirmed that this novel vaccinia virus strain still facilitated signal enhancement as detected by MRI and optoacoustic tomography. At the same time we demonstrated an enhanced oncolytic potential compared to the constitutively melanin synthesizing rVACV system.

Original languageEnglish
Pages (from-to)1045-1057
Number of pages13
Issue number10
StatePublished - 2015
Externally publishedYes


  • Molecular imaging
  • Oncolysis
  • Reporter gene
  • Virotherapy


Dive into the research topics of 'Doxycycline inducible melanogenic vaccinia virus as theranostic anti-cancer agent'. Together they form a unique fingerprint.

Cite this