Doxycycline inducible melanogenic vaccinia virus as theranostic anti-cancer agent

Lorenz Kirscher; Xosé Luis Deán-Ben; Miriam Scadeng; Angelika Zaremba; Qian Zhang; Christina Kober; Thomas Felix Fehm; Daniel Razansky; Vasilis Ntziachristos; Jochen Stritzker; Aladar A. Szalay

doi:10.7150/thno.12533

Doxycycline inducible melanogenic vaccinia virus as theranostic anti-cancer agent

Lorenz Kirscher, Xosé Luis Deán-Ben, Miriam Scadeng, Angelika Zaremba, Qian Zhang, Christina Kober, Thomas Felix Fehm, Daniel Razansky, Vasilis Ntziachristos, Jochen Stritzker, Aladar A. Szalay

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We reported earlier the diagnostic potential of a melanogenic vaccinia virus based system in magnetic resonance (MRI) and optoacoustic deep tissue imaging (MSOT). Since melanin overproduction lead to attenuated virus replication, we constructed a novel recombinant vaccinia virus strain (rVACV), GLV-1h462, which expressed the key enzyme of melanogenesis (tyrosinase) under the control of an inducible promoter-system. In this study melanin production was detected after exogenous addition of doxycycline in two different tumor xenograft mouse models. Furthermore, it was confirmed that this novel vaccinia virus strain still facilitated signal enhancement as detected by MRI and optoacoustic tomography. At the same time we demonstrated an enhanced oncolytic potential compared to the constitutively melanin synthesizing rVACV system.

Original language	English
Pages (from-to)	1045-1057
Number of pages	13
Journal	Theranostics
Volume	5
Issue number	10
DOIs	https://doi.org/10.7150/thno.12533
State	Published - 2015
Externally published	Yes

Keywords

Molecular imaging
Oncolysis
Reporter gene
Virotherapy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.7150/thno.12533

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@article{2b43ab6353b44098b060fefd1f289e02,

title = "Doxycycline inducible melanogenic vaccinia virus as theranostic anti-cancer agent",

abstract = "We reported earlier the diagnostic potential of a melanogenic vaccinia virus based system in magnetic resonance (MRI) and optoacoustic deep tissue imaging (MSOT). Since melanin overproduction lead to attenuated virus replication, we constructed a novel recombinant vaccinia virus strain (rVACV), GLV-1h462, which expressed the key enzyme of melanogenesis (tyrosinase) under the control of an inducible promoter-system. In this study melanin production was detected after exogenous addition of doxycycline in two different tumor xenograft mouse models. Furthermore, it was confirmed that this novel vaccinia virus strain still facilitated signal enhancement as detected by MRI and optoacoustic tomography. At the same time we demonstrated an enhanced oncolytic potential compared to the constitutively melanin synthesizing rVACV system.",

keywords = "Molecular imaging, Oncolysis, Reporter gene, Virotherapy",

author = "Lorenz Kirscher and De{\'a}n-Ben, {Xos{\'e} Luis} and Miriam Scadeng and Angelika Zaremba and Qian Zhang and Christina Kober and Fehm, {Thomas Felix} and Daniel Razansky and Vasilis Ntziachristos and Jochen Stritzker and Szalay, {Aladar A.}",

note = "Publisher Copyright: {\textcopyright} 2015 Ivyspring International Publisher.",

year = "2015",

doi = "10.7150/thno.12533",

language = "English",

volume = "5",

pages = "1045--1057",

journal = "Theranostics",

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TY - JOUR

T1 - Doxycycline inducible melanogenic vaccinia virus as theranostic anti-cancer agent

AU - Kirscher, Lorenz

AU - Deán-Ben, Xosé Luis

AU - Scadeng, Miriam

AU - Zaremba, Angelika

AU - Zhang, Qian

AU - Kober, Christina

AU - Fehm, Thomas Felix

AU - Razansky, Daniel

AU - Ntziachristos, Vasilis

AU - Stritzker, Jochen

AU - Szalay, Aladar A.

PY - 2015

Y1 - 2015

N2 - We reported earlier the diagnostic potential of a melanogenic vaccinia virus based system in magnetic resonance (MRI) and optoacoustic deep tissue imaging (MSOT). Since melanin overproduction lead to attenuated virus replication, we constructed a novel recombinant vaccinia virus strain (rVACV), GLV-1h462, which expressed the key enzyme of melanogenesis (tyrosinase) under the control of an inducible promoter-system. In this study melanin production was detected after exogenous addition of doxycycline in two different tumor xenograft mouse models. Furthermore, it was confirmed that this novel vaccinia virus strain still facilitated signal enhancement as detected by MRI and optoacoustic tomography. At the same time we demonstrated an enhanced oncolytic potential compared to the constitutively melanin synthesizing rVACV system.

AB - We reported earlier the diagnostic potential of a melanogenic vaccinia virus based system in magnetic resonance (MRI) and optoacoustic deep tissue imaging (MSOT). Since melanin overproduction lead to attenuated virus replication, we constructed a novel recombinant vaccinia virus strain (rVACV), GLV-1h462, which expressed the key enzyme of melanogenesis (tyrosinase) under the control of an inducible promoter-system. In this study melanin production was detected after exogenous addition of doxycycline in two different tumor xenograft mouse models. Furthermore, it was confirmed that this novel vaccinia virus strain still facilitated signal enhancement as detected by MRI and optoacoustic tomography. At the same time we demonstrated an enhanced oncolytic potential compared to the constitutively melanin synthesizing rVACV system.

KW - Molecular imaging

KW - Oncolysis

KW - Reporter gene

KW - Virotherapy

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U2 - 10.7150/thno.12533

DO - 10.7150/thno.12533

M3 - Article

C2 - 26199644

AN - SCOPUS:84945962074

SN - 1838-7640

VL - 5

SP - 1045

EP - 1057

JO - Theranostics

JF - Theranostics

IS - 10

ER -

Doxycycline inducible melanogenic vaccinia virus as theranostic anti-cancer agent

Abstract

Keywords

UN SDGs

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