Domibacillus robiginosus gen. nov., sp. nov., isolated from a pharmaceutical clean room

A novel red-pigmented bacterial strain, designated WS 4628^T, was isolated from a pharmaceutical clean room of a vaccine-producing company and was investigated in a taxonomic study using a polyphasic approach. The strain was Gram-stain-positive, strictly aerobic, motile, catalase-positive and produced spherical to slightly ellipsoidal endospores in rods. The genomic DNA G+C content was 44.1 mol%. The major fatty acids were anteiso-C_{15: 0}, iso-C_{15: 0} and anteiso-C_{17: 0} and the predominant quinone was MK-6. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid and an unidentified phospholipid. meso-diaminopimelic acid (type A1γ) was present in the cell-wall peptidoglycan and the major whole-cell sugars were glucose and ribose. The closest phylogenetic neighbours were identified as Bacillus badius ATCC 14574^T (95.8% 16S rRNA gene sequence similarity), Bacillus indicus Sd/3^T (94.8 %), Jeotgalibacillus alimentarius YKJ-13^T (94.8 %) and Bacillus cibi JG-30^T (94.8 %). Phylogenetic, physiological, biochemical and morphological differences between strain WS 4628^T and its closest relatives in the families Bacillaceae and Planococcaceae suggest that this strain represents a novel species in a new genus in the family Bacillaceae for which the name Domibacillus robiginosus gen. nov., sp. nov. is proposed; the type strain of the type species is WS 4628^T (=DSM 25058^T=LMG 26645^T).