DNA Structure-Specific Cleavage of DNA-Protein Crosslinks by the SPRTN Protease

Hannah K. Reinking; Hyun Seo Kang; Maximilian J. Götz; Hao Yi Li; Anja Kieser; Shubo Zhao; Aleida C. Acampora; Pedro Weickert; Evelyn Fessler; Lucas T. Jae; Michael Sattler; Julian Stingele

doi:10.1016/j.molcel.2020.08.003

DNA Structure-Specific Cleavage of DNA-Protein Crosslinks by the SPRTN Protease

Hannah K. Reinking
, Hyun Seo Kang
, Maximilian J. Götz
, Hao Yi Li
, Anja Kieser
, Shubo Zhao
, Aleida C. Acampora
, Pedro Weickert
, Evelyn Fessler
, Lucas T. Jae
, Michael Sattler
, Julian Stingele

Chair of Biomolecular NMR-Spectroscopy

University of Munich
Technical University of Munich
Helmholtz Zentrum München German Research Center for Environmental Health

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Reinking et al. show that the protease SPRTN degrades DNA-protein crosslinks in a DNA structure-specific manner, which restricts cleavage to biologically relevant scenarios. NMR analysis reveals that specificity is achieved by a bipartite strategy relying on two DNA binding interfaces that recognize single- and double-stranded features within the substrate.

Original language	English
Pages (from-to)	102-113.e6
Journal	Molecular Cell
Volume	80
Issue number	1
DOIs	https://doi.org/10.1016/j.molcel.2020.08.003
State	Published - 1 Oct 2020

Keywords

DNA repair
DNA structure
DNA-protein crosslink
SPRTN
Spartan
Wss1
formaldehyde
protease
topoisomerases

Access to Document

10.1016/j.molcel.2020.08.003

Cite this

@article{3d85457967974e7b8f79c43ccff2c6a9,

title = "DNA Structure-Specific Cleavage of DNA-Protein Crosslinks by the SPRTN Protease",

abstract = "Reinking et al. show that the protease SPRTN degrades DNA-protein crosslinks in a DNA structure-specific manner, which restricts cleavage to biologically relevant scenarios. NMR analysis reveals that specificity is achieved by a bipartite strategy relying on two DNA binding interfaces that recognize single- and double-stranded features within the substrate.",

keywords = "DNA repair, DNA structure, DNA-protein crosslink, SPRTN, Spartan, Wss1, formaldehyde, protease, topoisomerases",

author = "Reinking, \{Hannah K.\} and Kang, \{Hyun Seo\} and G{\"o}tz, \{Maximilian J.\} and Li, \{Hao Yi\} and Anja Kieser and Shubo Zhao and Acampora, \{Aleida C.\} and Pedro Weickert and Evelyn Fessler and Jae, \{Lucas T.\} and Michael Sattler and Julian Stingele",

note = "Publisher Copyright: {\textcopyright} 2020 The Authors",

year = "2020",

month = oct,

day = "1",

doi = "10.1016/j.molcel.2020.08.003",

language = "English",

volume = "80",

pages = "102--113.e6",

journal = "Molecular Cell",

issn = "1097-2765",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - DNA Structure-Specific Cleavage of DNA-Protein Crosslinks by the SPRTN Protease

AU - Reinking, Hannah K.

AU - Kang, Hyun Seo

AU - Götz, Maximilian J.

AU - Li, Hao Yi

AU - Kieser, Anja

AU - Zhao, Shubo

AU - Acampora, Aleida C.

AU - Weickert, Pedro

AU - Fessler, Evelyn

AU - Jae, Lucas T.

AU - Sattler, Michael

AU - Stingele, Julian

PY - 2020/10/1

Y1 - 2020/10/1

N2 - Reinking et al. show that the protease SPRTN degrades DNA-protein crosslinks in a DNA structure-specific manner, which restricts cleavage to biologically relevant scenarios. NMR analysis reveals that specificity is achieved by a bipartite strategy relying on two DNA binding interfaces that recognize single- and double-stranded features within the substrate.

AB - Reinking et al. show that the protease SPRTN degrades DNA-protein crosslinks in a DNA structure-specific manner, which restricts cleavage to biologically relevant scenarios. NMR analysis reveals that specificity is achieved by a bipartite strategy relying on two DNA binding interfaces that recognize single- and double-stranded features within the substrate.

KW - DNA repair

KW - DNA structure

KW - DNA-protein crosslink

KW - SPRTN

KW - Spartan

KW - Wss1

KW - formaldehyde

KW - protease

KW - topoisomerases

UR - https://www.scopus.com/pages/publications/85090490875

U2 - 10.1016/j.molcel.2020.08.003

DO - 10.1016/j.molcel.2020.08.003

M3 - Article

C2 - 32853547

AN - SCOPUS:85090490875

SN - 1097-2765

VL - 80

SP - 102-113.e6

JO - Molecular Cell

JF - Molecular Cell

IS - 1

ER -

DNA Structure-Specific Cleavage of DNA-Protein Crosslinks by the SPRTN Protease

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this