DNA Structure-Specific Cleavage of DNA-Protein Crosslinks by the SPRTN Protease

Hannah K. Reinking, Hyun Seo Kang, Maximilian J. Götz, Hao Yi Li, Anja Kieser, Shubo Zhao, Aleida C. Acampora, Pedro Weickert, Evelyn Fessler, Lucas T. Jae, Michael Sattler, Julian Stingele

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Reinking et al. show that the protease SPRTN degrades DNA-protein crosslinks in a DNA structure-specific manner, which restricts cleavage to biologically relevant scenarios. NMR analysis reveals that specificity is achieved by a bipartite strategy relying on two DNA binding interfaces that recognize single- and double-stranded features within the substrate.

Original languageEnglish
Pages (from-to)102-113.e6
JournalMolecular Cell
Volume80
Issue number1
DOIs
StatePublished - 1 Oct 2020

Keywords

  • DNA repair
  • DNA structure
  • DNA-protein crosslink
  • SPRTN
  • Spartan
  • Wss1
  • formaldehyde
  • protease
  • topoisomerases

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