Abstract
Reinking et al. show that the protease SPRTN degrades DNA-protein crosslinks in a DNA structure-specific manner, which restricts cleavage to biologically relevant scenarios. NMR analysis reveals that specificity is achieved by a bipartite strategy relying on two DNA binding interfaces that recognize single- and double-stranded features within the substrate.
Original language | English |
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Pages (from-to) | 102-113.e6 |
Journal | Molecular Cell |
Volume | 80 |
Issue number | 1 |
DOIs | |
State | Published - 1 Oct 2020 |
Keywords
- DNA repair
- DNA structure
- DNA-protein crosslink
- SPRTN
- Spartan
- Wss1
- formaldehyde
- protease
- topoisomerases