TY - JOUR
T1 - DNA polymorphism in morels
T2 - PCR/RFLP analysis of the ribosomal DNA spacers and microsatellite-primed PCR
AU - Buscot, François
AU - Wipf, Daniel
AU - Di Battista, Céline
AU - Munch, Jean Charles
AU - Botton, Bernard
AU - Martin, Francis
N1 - Funding Information:
This work was performed within the framework of the German-French co-operation project PROCOPE. F. Buscot is indebted to the program Eureka-Eurosilva for a research training grant allowing him to visit the INRA-Nancy (France) to learn the PCR methods. Best thanks also to Dr. C. Tebbe and to F. Schwieger for kindly providing research facilities at the FAL in Braunschweig (Germany) and to Dr Guy Costa (Microbiologie Forestikre INRA-Nancy) for his helpful comments on the use of the microsatellite-primed PCR. Herbert Boyle kindly revised the language.
PY - 1996/1
Y1 - 1996/1
N2 - As a part of investigations on heterokaryon formation in morels, a characterization of DNA polymorphism within this fungal group was attempted. In order to assess which discrimination level is necessary to trace nucleus populations in heterokaryons, but also in the context of the debatable species definition in morels, different taxa and strain types (monosporal and heterokaryons) were analysed with two polymerase chain reaction (PCR) techniques of distinct sensitivity: (i) PCR of the internal transcribed spacer (ITS) and the intergenic spacer (IGS) of the ribosomal nuclear DNA coupled with restriction fragment length polymorphism (RFLP) analysis, (ii) microsatellite-primed PCR. The ITS and IGS PCR/RFLP appeared at first to be adequate to assess morel systematics. The microsatellite-primed PCR with the primer (GTG)5 revealed, however, that morels exhibit less intraspecific DNA polymorphism than other ascomycetes. Based upon these results, two strategies for investigating somatic strain interactions within morels using DNA analyses are proposed.
AB - As a part of investigations on heterokaryon formation in morels, a characterization of DNA polymorphism within this fungal group was attempted. In order to assess which discrimination level is necessary to trace nucleus populations in heterokaryons, but also in the context of the debatable species definition in morels, different taxa and strain types (monosporal and heterokaryons) were analysed with two polymerase chain reaction (PCR) techniques of distinct sensitivity: (i) PCR of the internal transcribed spacer (ITS) and the intergenic spacer (IGS) of the ribosomal nuclear DNA coupled with restriction fragment length polymorphism (RFLP) analysis, (ii) microsatellite-primed PCR. The ITS and IGS PCR/RFLP appeared at first to be adequate to assess morel systematics. The microsatellite-primed PCR with the primer (GTG)5 revealed, however, that morels exhibit less intraspecific DNA polymorphism than other ascomycetes. Based upon these results, two strategies for investigating somatic strain interactions within morels using DNA analyses are proposed.
UR - http://www.scopus.com/inward/record.url?scp=0030062449&partnerID=8YFLogxK
U2 - 10.1016/S0953-7562(96)80101-8
DO - 10.1016/S0953-7562(96)80101-8
M3 - Article
AN - SCOPUS:0030062449
SN - 0953-7562
VL - 100
SP - 63
EP - 71
JO - Mycological Research
JF - Mycological Research
IS - 1
ER -