TY - JOUR
T1 - DNA flow cytometry in stage IB and II cervical carcinoma
AU - Pfisterer, J.
AU - Kommoss, F.
AU - Sauerbrei, W.
AU - Baranski, B.
AU - Kiechle, M.
AU - Ikenberg, H.
AU - Du Bois, A.
AU - Pfleiderer, A.
PY - 1996
Y1 - 1996
N2 - In a retrospective study the prognostic significance of nuclear DNA content was investigated, as measured by flow cytometry, of the tumor specimens from 212 women with nonpretreated FIGO stage IB and II cervical cancer. One-hundred and thirty cases (62%) were found to be diploid, whereas 82 (38%) were aneuploid. Univariate analysis of the follow-up data showed an increased relative risk (RR) for recurrence free survival (RFS) for stage II tumors (RR = 1.87, 95% CI: 1.13-3.10, P = 0.015) and for age (RR = 1.52, 95% CI: 0.66-3.52 and RR = 2.35, 95% CI: 1.19-4.65, P = 0.032). Ploidy showed a relative risk of 1.33 (95% CI: 0.83-2.13, NS). In addition, univariate analysis of overall survival (OS) revealed similar results. For the subgroup of patients with primary surgery (n = 151), positive pelvic nodes (RR = 5.38, 95% CI: 2.70-10.71, P = 0.0001) and parametrial extension (RR = 2.53, 95% CI: 1.24-5.17, P = 0.011) were significant factors for OS after univariate analysis, the estimated effects on RFS were slightly smaller. Multivariate analysis of RFS for the whole study population showed age, histologic grade and stage with a slightly increased risk, but no effect was significant. Ploidy with an RR of 0.97 (95% CI: 0.58-1.62) seems to have no influence on prognosis. For the subgroup with primary surgery, ploidy again failed statistical significance with an RR of 1.20 (95% CI: 0.58-2.49). Our results suggest that abnormalities of the nuclear DNA content in this homogeneous group of patients are associated with clinical and morphological prognosticators, however, ploidy is not an independent prognostic factor for RFS, or for the whole study population or for the subgroup with primary surgery.
AB - In a retrospective study the prognostic significance of nuclear DNA content was investigated, as measured by flow cytometry, of the tumor specimens from 212 women with nonpretreated FIGO stage IB and II cervical cancer. One-hundred and thirty cases (62%) were found to be diploid, whereas 82 (38%) were aneuploid. Univariate analysis of the follow-up data showed an increased relative risk (RR) for recurrence free survival (RFS) for stage II tumors (RR = 1.87, 95% CI: 1.13-3.10, P = 0.015) and for age (RR = 1.52, 95% CI: 0.66-3.52 and RR = 2.35, 95% CI: 1.19-4.65, P = 0.032). Ploidy showed a relative risk of 1.33 (95% CI: 0.83-2.13, NS). In addition, univariate analysis of overall survival (OS) revealed similar results. For the subgroup of patients with primary surgery (n = 151), positive pelvic nodes (RR = 5.38, 95% CI: 2.70-10.71, P = 0.0001) and parametrial extension (RR = 2.53, 95% CI: 1.24-5.17, P = 0.011) were significant factors for OS after univariate analysis, the estimated effects on RFS were slightly smaller. Multivariate analysis of RFS for the whole study population showed age, histologic grade and stage with a slightly increased risk, but no effect was significant. Ploidy with an RR of 0.97 (95% CI: 0.58-1.62) seems to have no influence on prognosis. For the subgroup with primary surgery, ploidy again failed statistical significance with an RR of 1.20 (95% CI: 0.58-2.49). Our results suggest that abnormalities of the nuclear DNA content in this homogeneous group of patients are associated with clinical and morphological prognosticators, however, ploidy is not an independent prognostic factor for RFS, or for the whole study population or for the subgroup with primary surgery.
KW - DNA content
KW - cervical carcinoma
KW - flow cytometry
KW - ploidy
KW - prognostic factors
UR - http://www.scopus.com/inward/record.url?scp=0029919842&partnerID=8YFLogxK
U2 - 10.1046/j.1525-1438.1996.06010054.x
DO - 10.1046/j.1525-1438.1996.06010054.x
M3 - Article
AN - SCOPUS:0029919842
SN - 1048-891X
VL - 6
SP - 54
EP - 60
JO - International Journal of Gynecological Cancer
JF - International Journal of Gynecological Cancer
IS - 1
ER -