TY - JOUR
T1 - Divergence of evolutionary ways among common sym genes
T2 - CASTOR and CCaMK show functional conservation between two symbiosis systems and constitute the root of a common signaling pathway
AU - Banba, Mari
AU - Gutjahr, Caroline
AU - Miyao, Akio
AU - Hirochika, Hirohiko
AU - Paszkowski, Uta
AU - Kouchi, Hiroshi
AU - Imaizumi-Anraku, Haruko
N1 - Funding Information:
The Ministry of Agriculture, Forestry, and Fisheries of Japan (Rice Genome Project Grant PMI-0001); the Program of Basic Research Activities for Innovative Biosciences (BRAIN).
PY - 2008/11
Y1 - 2008/11
N2 - In recent years a number of legume genes involved in root nodule (RN) symbiosis have been identified in the model legumes, Lotus japonicus (Lotus) and Medicago truncatula. Among them, a distinct set of genes has been categorized as a common symbiosis pathway (CSP), because they are also essential for another mutual interaction, the arbuscular mycorrhiza (AM) symbiosis, which is evolutionarily older than the RN symbiosis and is widely distributed in the plant kingdom. Based on the concept that the legume RN symbiosis has evolved from the ancient AM symbiosis, one issue is whether the CSP is functionally conserved between non-nodulating plants, such as rice, and nodulating legumes. We identified three rice CSP gene orthologs, OsCASTOR, OsPOLLUX and OsCCaMK, and demonstrated the indispensable roles of OsPOLLUX and OsCCaMK in rice AM symbiosis. Interestingly, molecular transfection of either OsCASTOR or OsCCaMK could fully complement symbiosis defects in the corresponding Lotus mutant lines for both the AM and RN symbioses. Our results not only provide a conserved genetic basis for the AM symbiosis between rice and Lotus, but also indicate that the core of the CSP has been well conserved during the evolution of RN symbiosis. Through evolution, CASTOR and CCaMK have remained as the molecular basis for the maintenance of CSP functions in the two symbiosis systems.
AB - In recent years a number of legume genes involved in root nodule (RN) symbiosis have been identified in the model legumes, Lotus japonicus (Lotus) and Medicago truncatula. Among them, a distinct set of genes has been categorized as a common symbiosis pathway (CSP), because they are also essential for another mutual interaction, the arbuscular mycorrhiza (AM) symbiosis, which is evolutionarily older than the RN symbiosis and is widely distributed in the plant kingdom. Based on the concept that the legume RN symbiosis has evolved from the ancient AM symbiosis, one issue is whether the CSP is functionally conserved between non-nodulating plants, such as rice, and nodulating legumes. We identified three rice CSP gene orthologs, OsCASTOR, OsPOLLUX and OsCCaMK, and demonstrated the indispensable roles of OsPOLLUX and OsCCaMK in rice AM symbiosis. Interestingly, molecular transfection of either OsCASTOR or OsCCaMK could fully complement symbiosis defects in the corresponding Lotus mutant lines for both the AM and RN symbioses. Our results not only provide a conserved genetic basis for the AM symbiosis between rice and Lotus, but also indicate that the core of the CSP has been well conserved during the evolution of RN symbiosis. Through evolution, CASTOR and CCaMK have remained as the molecular basis for the maintenance of CSP functions in the two symbiosis systems.
KW - Arbuscular mycorrhizal symbiosis
KW - Common symbiosis pathway
KW - Lotus japonicus
KW - Oryza sativa
KW - Rice
KW - Root nodule symbiosis
UR - http://www.scopus.com/inward/record.url?scp=56449127013&partnerID=8YFLogxK
U2 - 10.1093/pcp/pcn153
DO - 10.1093/pcp/pcn153
M3 - Article
C2 - 18852152
AN - SCOPUS:56449127013
SN - 0032-0781
VL - 49
SP - 1659
EP - 1671
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
IS - 11
ER -