Dissimilar kinetic behavior of electrically manipulated single- and double-stranded DNA tethered to a gold surface

Ulrich Rant; Kenji Arinaga; Marc Tornow; Woon Kim Yong; Roland R. Netz; Shozo Fujita; Naoki Yokoyama; Gerhard Abstreiter

doi:10.1529/biophysj.105.078857

Dissimilar kinetic behavior of electrically manipulated single- and double-stranded DNA tethered to a gold surface

Ulrich Rant, Kenji Arinaga, Marc Tornow, Woon Kim Yong, Roland R. Netz, Shozo Fujita, Naoki Yokoyama, Gerhard Abstreiter

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

We report on the electrical manipulation of single- and double-stranded oligodeoxynucleotides that are end tethered to gold surfaces in electrolyte solution. The response to alternating repulsive and attractive electric surface fields is studied by time-resolved fluorescence measurements, revealing markedly distinct dynamics for the flexible single-stranded and stiff double-stranded DNA, respectively. Hydrodynamic simulations rationalize this finding and disclose two different kinetic mechanisms: stiff polymers undergo rotation around the anchoring pivot point; flexible polymers, on the other hand, are pulled onto the attracting surface segment by segment.

Original language	English
Pages (from-to)	3666-3671
Number of pages	6
Journal	Biophysical Journal
Volume	90
Issue number	10
DOIs	https://doi.org/10.1529/biophysj.105.078857
State	Published - May 2006

Access to Document

10.1529/biophysj.105.078857

Cite this

@article{2631421b84b6401cb61170c9dea56126,

title = "Dissimilar kinetic behavior of electrically manipulated single- and double-stranded DNA tethered to a gold surface",

abstract = "We report on the electrical manipulation of single- and double-stranded oligodeoxynucleotides that are end tethered to gold surfaces in electrolyte solution. The response to alternating repulsive and attractive electric surface fields is studied by time-resolved fluorescence measurements, revealing markedly distinct dynamics for the flexible single-stranded and stiff double-stranded DNA, respectively. Hydrodynamic simulations rationalize this finding and disclose two different kinetic mechanisms: stiff polymers undergo rotation around the anchoring pivot point; flexible polymers, on the other hand, are pulled onto the attracting surface segment by segment.",

author = "Ulrich Rant and Kenji Arinaga and Marc Tornow and Yong, {Woon Kim} and Netz, {Roland R.} and Shozo Fujita and Naoki Yokoyama and Gerhard Abstreiter",

year = "2006",

month = may,

doi = "10.1529/biophysj.105.078857",

language = "English",

volume = "90",

pages = "3666--3671",

journal = "Biophysical Journal",

issn = "0006-3495",

publisher = "Elsevier B.V.",

number = "10",

}

TY - JOUR

T1 - Dissimilar kinetic behavior of electrically manipulated single- and double-stranded DNA tethered to a gold surface

AU - Rant, Ulrich

AU - Arinaga, Kenji

AU - Tornow, Marc

AU - Yong, Woon Kim

AU - Netz, Roland R.

AU - Fujita, Shozo

AU - Yokoyama, Naoki

AU - Abstreiter, Gerhard

PY - 2006/5

Y1 - 2006/5

N2 - We report on the electrical manipulation of single- and double-stranded oligodeoxynucleotides that are end tethered to gold surfaces in electrolyte solution. The response to alternating repulsive and attractive electric surface fields is studied by time-resolved fluorescence measurements, revealing markedly distinct dynamics for the flexible single-stranded and stiff double-stranded DNA, respectively. Hydrodynamic simulations rationalize this finding and disclose two different kinetic mechanisms: stiff polymers undergo rotation around the anchoring pivot point; flexible polymers, on the other hand, are pulled onto the attracting surface segment by segment.

AB - We report on the electrical manipulation of single- and double-stranded oligodeoxynucleotides that are end tethered to gold surfaces in electrolyte solution. The response to alternating repulsive and attractive electric surface fields is studied by time-resolved fluorescence measurements, revealing markedly distinct dynamics for the flexible single-stranded and stiff double-stranded DNA, respectively. Hydrodynamic simulations rationalize this finding and disclose two different kinetic mechanisms: stiff polymers undergo rotation around the anchoring pivot point; flexible polymers, on the other hand, are pulled onto the attracting surface segment by segment.

UR - http://www.scopus.com/inward/record.url?scp=33646188309&partnerID=8YFLogxK

U2 - 10.1529/biophysj.105.078857

DO - 10.1529/biophysj.105.078857

M3 - Article

C2 - 16473909

AN - SCOPUS:33646188309

SN - 0006-3495

VL - 90

SP - 3666

EP - 3671

JO - Biophysical Journal

JF - Biophysical Journal

IS - 10

ER -

Dissimilar kinetic behavior of electrically manipulated single- and double-stranded DNA tethered to a gold surface

Abstract

Access to Document

Other files and links

Fingerprint

Cite this