Dissecting the genetic basis of resistance to malaria parasites in Anopheles gambiae

Stephanie A. Blandin; Rui Wang-Sattler; Marina Lamacchia; Julien Gagneur; Gareth Lycett; Ye Ning; Elena A. Levashina; Lars M. Steinmetz

doi:10.1126/science.1175241

Dissecting the genetic basis of resistance to malaria parasites in Anopheles gambiae

Stephanie A. Blandin, Rui Wang-Sattler, Marina Lamacchia, Julien Gagneur, Gareth Lycett, Ye Ning, Elena A. Levashina, Lars M. Steinmetz

Research output: Contribution to journal › Article › peer-review

104 Scopus citations

Abstract

The ability of Anopheles gambiae mosquitoes to transmit Plasmodium parasites is highly variable between individuals. However, the genetic basis of this variability has remained unknown. We combined genome-wide mapping and reciprocal allele-specific RNA interference (rasRNAi) to identify the genomic locus that confers resistance to malaria parasites and demonstrated that polymorphisms in a single gene encoding the antiparasitic thioester-containing protein 1 (TEPl) explain a substantial part of the variability in parasite killing. The link between TEP1 alleles and resistance to malaria may offer new tools for controlling malaria transmission. The successful application of rasRNAi in Anopheles suggests that it could also be applied to other organisms where RNAi is feasible to dissect complex phenotypes to the level of individual quantitative trait alleles.

Original language	English
Pages (from-to)	147-150
Number of pages	4
Journal	Science
Volume	326
Issue number	5949
DOIs	https://doi.org/10.1126/science.1175241
State	Published - 2009
Externally published	Yes

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abstract = "The ability of Anopheles gambiae mosquitoes to transmit Plasmodium parasites is highly variable between individuals. However, the genetic basis of this variability has remained unknown. We combined genome-wide mapping and reciprocal allele-specific RNA interference (rasRNAi) to identify the genomic locus that confers resistance to malaria parasites and demonstrated that polymorphisms in a single gene encoding the antiparasitic thioester-containing protein 1 (TEPl) explain a substantial part of the variability in parasite killing. The link between TEP1 alleles and resistance to malaria may offer new tools for controlling malaria transmission. The successful application of rasRNAi in Anopheles suggests that it could also be applied to other organisms where RNAi is feasible to dissect complex phenotypes to the level of individual quantitative trait alleles.",

author = "Blandin, {Stephanie A.} and Rui Wang-Sattler and Marina Lamacchia and Julien Gagneur and Gareth Lycett and Ye Ning and Levashina, {Elena A.} and Steinmetz, {Lars M.}",

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AU - Blandin, Stephanie A.

AU - Wang-Sattler, Rui

AU - Lamacchia, Marina

AU - Gagneur, Julien

AU - Lycett, Gareth

AU - Ning, Ye

AU - Levashina, Elena A.

AU - Steinmetz, Lars M.

PY - 2009

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AB - The ability of Anopheles gambiae mosquitoes to transmit Plasmodium parasites is highly variable between individuals. However, the genetic basis of this variability has remained unknown. We combined genome-wide mapping and reciprocal allele-specific RNA interference (rasRNAi) to identify the genomic locus that confers resistance to malaria parasites and demonstrated that polymorphisms in a single gene encoding the antiparasitic thioester-containing protein 1 (TEPl) explain a substantial part of the variability in parasite killing. The link between TEP1 alleles and resistance to malaria may offer new tools for controlling malaria transmission. The successful application of rasRNAi in Anopheles suggests that it could also be applied to other organisms where RNAi is feasible to dissect complex phenotypes to the level of individual quantitative trait alleles.

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Dissecting the genetic basis of resistance to malaria parasites in Anopheles gambiae

Abstract

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