Abstract
Rapid advances in live-cell imaging have now enabled direct observation of the transcription of single nascent mRNA molecules from an endogenous yeast gene. A novel quantitative fluctuation analysis of fluorescently labeled mRNA revealed the kinetics of transcription initiation and the dynamics of elongation and termination (see picture; GFP=green fluorescent protein, PP7 is a bacteriophage coat protein, RNAPII=RNA polymerase II, TF=transcription factor).
Original language | English |
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Pages (from-to) | 9788-9790 |
Number of pages | 3 |
Journal | Angewandte Chemie International Edition in English |
Volume | 50 |
Issue number | 42 |
DOIs | |
State | Published - 10 Oct 2011 |
Externally published | Yes |
Keywords
- diffusion
- fluorescence correlation spectroscopy
- single-cell biology
- single-molecule studies
- transcription