Direct observation of single RNA polymerase processing through a single endogenous gene in a living yeast cell

Barbara Treutlein, Jens Michaelis

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Rapid advances in live-cell imaging have now enabled direct observation of the transcription of single nascent mRNA molecules from an endogenous yeast gene. A novel quantitative fluctuation analysis of fluorescently labeled mRNA revealed the kinetics of transcription initiation and the dynamics of elongation and termination (see picture; GFP=green fluorescent protein, PP7 is a bacteriophage coat protein, RNAPII=RNA polymerase II, TF=transcription factor).

Original languageEnglish
Pages (from-to)9788-9790
Number of pages3
JournalAngewandte Chemie International Edition in English
Volume50
Issue number42
DOIs
StatePublished - 10 Oct 2011
Externally publishedYes

Keywords

  • diffusion
  • fluorescence correlation spectroscopy
  • single-cell biology
  • single-molecule studies
  • transcription

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