TY - JOUR
T1 - Direct effect of CD on glutathione S-transferase and glutathione reductase from Calystegia sepium
AU - Lyubenova, Lyudmila
AU - Götz, Christine
AU - Golan-Goldhirsh, Avi
AU - Schröder, Peter
N1 - Funding Information:
The authors thank Frank Laturnus, Linköpings Universitet Sweden, for critically reading the manuscript. Funding for Lyudmila Lyubenova by a grant from the Bavarian State Ministry for Education and Arts in the frame of the BAYHOST program is gratefully accepted.
PY - 2007/11
Y1 - 2007/11
N2 - Interactions between heavy metals, glutathione, glutathione S-transferase (GST), and glutathione reductase (GR) are being investigated by many working groups, but evaluation of the direct effect of Cd2+ on these enzymes in vitro is lacking. We report here the effect of cadmium (10, 50, 100, 250 μM CdSO4) on partially purified enzymes from Calystegia sepium. Plants were grown under normal field conditions without metals and the enzymes were extracted by Tris buffer and partially purified by ammonium sulphate fractionation and gel filtration. Glutathione S-transferase activity was measured with different substrates, i.e., 1-chloro-2,4-dinitrobenzene (CDNB), p-nitrobenzylchloride (NBC), and the herbicide Fluorodifen. GST activity was significantly lower in leaf compared to stem, flower, and rhizome and the inhibitory effect of Cd was obtained with NBC and Fluorodifen substrates at 250 μM. There was no effect of Cd on GR activity up to 250 μM.
AB - Interactions between heavy metals, glutathione, glutathione S-transferase (GST), and glutathione reductase (GR) are being investigated by many working groups, but evaluation of the direct effect of Cd2+ on these enzymes in vitro is lacking. We report here the effect of cadmium (10, 50, 100, 250 μM CdSO4) on partially purified enzymes from Calystegia sepium. Plants were grown under normal field conditions without metals and the enzymes were extracted by Tris buffer and partially purified by ammonium sulphate fractionation and gel filtration. Glutathione S-transferase activity was measured with different substrates, i.e., 1-chloro-2,4-dinitrobenzene (CDNB), p-nitrobenzylchloride (NBC), and the herbicide Fluorodifen. GST activity was significantly lower in leaf compared to stem, flower, and rhizome and the inhibitory effect of Cd was obtained with NBC and Fluorodifen substrates at 250 μM. There was no effect of Cd on GR activity up to 250 μM.
KW - Calystegia sepium
KW - CdSO cadmium
KW - Glutathione
KW - Glutathione S-transferase
KW - Heavy metals
UR - http://www.scopus.com/inward/record.url?scp=36849020079&partnerID=8YFLogxK
U2 - 10.1080/15226510701709556
DO - 10.1080/15226510701709556
M3 - Article
C2 - 18246773
AN - SCOPUS:36849020079
SN - 1522-6514
VL - 9
SP - 465
EP - 473
JO - International Journal of Phytoremediation
JF - International Journal of Phytoremediation
IS - 6
ER -