Dip-and-read test strips for the determination of trinitrotoluene (TNT) in drinking water

Carola Heiss; Michael G. Weller; Reinhard Niessner

doi:10.1016/S0003-2670(99)00422-5

Dip-and-read test strips for the determination of trinitrotoluene (TNT) in drinking water

Carola Heiss, Michael G. Weller, Reinhard Niessner

Natural Sciences

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

A test strip to quantify trinitrotoluene (TNT) in water has been developed using a homogenous apoenzyme reactivation immunoassay system (ARIS). In comparison with other test kits for the detection of TNT, the novel test strip is much simpler in application. The test strip has only to be dipped into the aqueous sample. A blue color develops on the reagent strips proportional to the TNT concentration. The concentration of TNT is determined either by visual comparison with a color card, or more precisely using a reflectometer. A measuring range of about 1-10,000μgl^-1 TNT in water was demonstrated. The reagent strips were prepared by sequential impregnation of a cellulose pad with two solutions, one based on acetone and the other based on aqueous buffer. All necessary components are included in the reagent strip in dry form. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	309-316
Number of pages	8
Journal	Analytica Chimica Acta
Volume	396
Issue number	2-3
DOIs	https://doi.org/10.1016/S0003-2670(99)00422-5
State	Published - 20 Sep 1999

Keywords

Drinking water
Enzyme stabilization
Homogeneous enzyme immunoassay
Test strips
Trinitrotoluene

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10.1016/S0003-2670(99)00422-5

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title = "Dip-and-read test strips for the determination of trinitrotoluene (TNT) in drinking water",

abstract = "A test strip to quantify trinitrotoluene (TNT) in water has been developed using a homogenous apoenzyme reactivation immunoassay system (ARIS). In comparison with other test kits for the detection of TNT, the novel test strip is much simpler in application. The test strip has only to be dipped into the aqueous sample. A blue color develops on the reagent strips proportional to the TNT concentration. The concentration of TNT is determined either by visual comparison with a color card, or more precisely using a reflectometer. A measuring range of about 1-10,000μgl-1 TNT in water was demonstrated. The reagent strips were prepared by sequential impregnation of a cellulose pad with two solutions, one based on acetone and the other based on aqueous buffer. All necessary components are included in the reagent strip in dry form. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "Drinking water, Enzyme stabilization, Homogeneous enzyme immunoassay, Test strips, Trinitrotoluene",

author = "Carola Heiss and Weller, {Michael G.} and Reinhard Niessner",

note = "Funding Information: We would like to thank Merck KGaA, Darmstadt, for financial support. In addition, we kindly want to thank Dr. A.F. B{\"u}ckmann, Gesellschaft f{\"u}r Biotechnologische Forschung mbH (GBF), Braunschweig, for donating the precursor of the FAD–TNT conjugate. Furthermore, the gift of monoclonal antibody against TNT from Strategic Diagnostics Inc., Newark, USA is gratefully acknowledged.",

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doi = "10.1016/S0003-2670(99)00422-5",

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TY - JOUR

T1 - Dip-and-read test strips for the determination of trinitrotoluene (TNT) in drinking water

AU - Heiss, Carola

AU - Weller, Michael G.

AU - Niessner, Reinhard

N1 - Funding Information: We would like to thank Merck KGaA, Darmstadt, for financial support. In addition, we kindly want to thank Dr. A.F. Bückmann, Gesellschaft für Biotechnologische Forschung mbH (GBF), Braunschweig, for donating the precursor of the FAD–TNT conjugate. Furthermore, the gift of monoclonal antibody against TNT from Strategic Diagnostics Inc., Newark, USA is gratefully acknowledged.

PY - 1999/9/20

Y1 - 1999/9/20

N2 - A test strip to quantify trinitrotoluene (TNT) in water has been developed using a homogenous apoenzyme reactivation immunoassay system (ARIS). In comparison with other test kits for the detection of TNT, the novel test strip is much simpler in application. The test strip has only to be dipped into the aqueous sample. A blue color develops on the reagent strips proportional to the TNT concentration. The concentration of TNT is determined either by visual comparison with a color card, or more precisely using a reflectometer. A measuring range of about 1-10,000μgl-1 TNT in water was demonstrated. The reagent strips were prepared by sequential impregnation of a cellulose pad with two solutions, one based on acetone and the other based on aqueous buffer. All necessary components are included in the reagent strip in dry form. Copyright (C) 1999 Elsevier Science B.V.

AB - A test strip to quantify trinitrotoluene (TNT) in water has been developed using a homogenous apoenzyme reactivation immunoassay system (ARIS). In comparison with other test kits for the detection of TNT, the novel test strip is much simpler in application. The test strip has only to be dipped into the aqueous sample. A blue color develops on the reagent strips proportional to the TNT concentration. The concentration of TNT is determined either by visual comparison with a color card, or more precisely using a reflectometer. A measuring range of about 1-10,000μgl-1 TNT in water was demonstrated. The reagent strips were prepared by sequential impregnation of a cellulose pad with two solutions, one based on acetone and the other based on aqueous buffer. All necessary components are included in the reagent strip in dry form. Copyright (C) 1999 Elsevier Science B.V.

KW - Drinking water

KW - Enzyme stabilization

KW - Homogeneous enzyme immunoassay

KW - Test strips

KW - Trinitrotoluene

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U2 - 10.1016/S0003-2670(99)00422-5

DO - 10.1016/S0003-2670(99)00422-5

M3 - Article

AN - SCOPUS:0032862203

SN - 0003-2670

VL - 396

SP - 309

EP - 316

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 2-3

ER -

Dip-and-read test strips for the determination of trinitrotoluene (TNT) in drinking water

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