DiMmer: Discovery of differentially methylated regions in epigenome-wide association study (EWAS) data

Tobias Frisch, Jonatan Gøttcke, Richard Röttger, Qihua Tan, Jan Baumbach

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

DNA-methylation has a strong influence on gene expression such that differences in methylation are associated with a wide range of diseases. Array-based approaches like the Illumina 450 K or 850 K EPIC chips have been used in a wide range of studies mostly comparing a disease group with healthy control, but also to correlate with survival times, for instance. Processing, normalization, and analysis of raw data require extensive knowledge in statistics and programming languages such as R. Here we introduce DiMmer, an easy-to-use Java tool for the analysis of EWAS. A graphical user interface guides the user through preprocessing, normalization, testing for differentially methylated CpGs, and finally the discovery of differentially methylated regions (DMRs). The software performs randomization tests to compute empirical P-values, corrects for multiple testing, and requires no prior knowledge in programming. All computed results are provided as plots or tables and can be easily exported. DiMmer is thus a powerful one-stop-shop for EWAS data analysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages51-62
Number of pages12
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1807
ISSN (Print)1064-3745

Keywords

  • DNA modification
  • Differentially methylated regions
  • Epigenetic
  • Epigenome-wide association studies
  • Methylation

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