DIGGER 2.0: Digging into the functional impact of differential splicing on human and mouse disorders

Elias Albrecht; Konstantin Pelz; Alexander Gress; Hieu Nguyen Trung; Olga V. Kalinina; Tim Kacprowski; Jan Baumbach; Markus List; Olga Tsoy

doi:10.1093/nar/gkaf384

DIGGER 2.0: Digging into the functional impact of differential splicing on human and mouse disorders

Elias Albrecht
, Konstantin Pelz
, Alexander Gress
, Hieu Nguyen Trung
, Olga V. Kalinina
, Tim Kacprowski
, Jan Baumbach
, Markus List
, Olga Tsoy

Assistant Professorship of Data Science in Systems Biology

Technical University of Munich
Universität Hamburg
Helmholtz Centre for Infection Research (HZI)
Saarland University
Saarland University Medical Center
Medizinische Hochschule Hannover
Technische Universität Braunschweig
University of Southern Denmark

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Changes in alternative splicing between groups or conditions contribute to protein-protein interaction rewiring, a consequence often neglected in data analysis. The web server and database DIGGER overcomes this limitation by augmenting a protein-protein interaction network with domain-domain interactions and splicing information. Here, we present DIGGER 2.0, which now features both experimental and newly added predicted domain-domain interactions. In addition to the human interactome, DIGGER 2.0 adds support for mouse as an important model organism. Additionally, we integrated the splicing analysis tool NEASE, which allows users to perform online splicing- and interactome-informed enrichment analysis on RNA-seq data. In two application cases (multiple sclerosis and mice models of cardiac diseases), we show the utility of DIGGER 2.0 for deeper exploration and functional interpretation of changes in alternative splicing in human and mouse disorders. DIGGER 2.0 is available at https://exbio.wzw.tum.de/digger/.

Original language	English
Pages (from-to)	W245-W252
Journal	Nucleic Acids Research
Volume	53
Issue number	W1
DOIs	https://doi.org/10.1093/nar/gkaf384
State	Published - 7 Jul 2025

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title = "DIGGER 2.0: Digging into the functional impact of differential splicing on human and mouse disorders",

abstract = "Changes in alternative splicing between groups or conditions contribute to protein-protein interaction rewiring, a consequence often neglected in data analysis. The web server and database DIGGER overcomes this limitation by augmenting a protein-protein interaction network with domain-domain interactions and splicing information. Here, we present DIGGER 2.0, which now features both experimental and newly added predicted domain-domain interactions. In addition to the human interactome, DIGGER 2.0 adds support for mouse as an important model organism. Additionally, we integrated the splicing analysis tool NEASE, which allows users to perform online splicing- and interactome-informed enrichment analysis on RNA-seq data. In two application cases (multiple sclerosis and mice models of cardiac diseases), we show the utility of DIGGER 2.0 for deeper exploration and functional interpretation of changes in alternative splicing in human and mouse disorders. DIGGER 2.0 is available at https://exbio.wzw.tum.de/digger/.",

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AU - Albrecht, Elias

AU - Pelz, Konstantin

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AU - Trung, Hieu Nguyen

AU - Kalinina, Olga V.

AU - Kacprowski, Tim

AU - Baumbach, Jan

AU - List, Markus

AU - Tsoy, Olga

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DIGGER 2.0: Digging into the functional impact of differential splicing on human and mouse disorders

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