Differentiated human adipose-derived stromal cells exhibit the phenotypic and functional characteristics of mature Schwann cells through a modified approach

Yu Kang, Yutian Liu, Zhenyu Liu, Sen Ren, Hewei Xiong, Jing Chen, Dominik Duscher, Hans Günther Machens, Wei Liu, Guojun Guo, Peng Zhan, Hongrui Chen, Zhenbing Chen

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Background aims: Tissue engineering technology is a promising therapeutic strategy in peripheral nerve injury. Schwann cells (SCs) are deemed to be a vital component of cell-based nerve regeneration therapies. Many methods for producing SC-like cells derived from adipose-derived stromal cells (ADSCs) have been explored, but their phenotypic and functional characteristics remain unsatisfactory. Methods: We investigated whether human ADSCs can be induced to differentiate into mature and stable SC-like cells with the addition of insulin, progestero``ne and glucocorticoids. The phenotypic and functional characteristics of new differentiated ADSCs (modified SC-like cells) were evaluated by real-time quantitative polymerase chain reaction, enzyme-linked immunosorbent assay and immunocytochemistry in vitro. Cells loaded into collagen sponge biomaterials were implanted around transected sciatic nerves with a 10-mm gap in vivo. The axon regrowth and functional recovery of the regenerated nerves were assessed by immunohistochemistry and Walking footprint analysis. Results: After differentiation induction, the modified SC-like cells showed significantly up-regulated levels of S100B and P0 and enhanced proliferative and migratory capacities. In addition, the modified SC-like cells showed increased secretion of neurotrophic factors, and their functional characteristics were maintained for more than 3 weeks after removing the induction reagents. The modified SC-like cells exhibited significantly enhanced axon regrowth, myelination and functional recovery after sciatic nerve injury. Conclusions: Overall, the results suggest that this modified induction method can induce human ADSCs to differentiate into cells with the molecular and functional properties of mature SCs and increase the promotion of peripheral nerve regeneration.

Original languageEnglish
Pages (from-to)987-1003
Number of pages17
JournalCytotherapy
Volume21
Issue number9
DOIs
StatePublished - Sep 2019
Externally publishedYes

Keywords

  • Schwann cell–like cells
  • adipose-derived stromal cells
  • differentiation induction
  • peripheral nerve regeneration
  • tissue engineering

Fingerprint

Dive into the research topics of 'Differentiated human adipose-derived stromal cells exhibit the phenotypic and functional characteristics of mature Schwann cells through a modified approach'. Together they form a unique fingerprint.

Cite this