TY - JOUR
T1 - Diet-induced and mono-genetic obesity alter volatile organic compound signature in mice
AU - Kistler, Martin
AU - Muntean, Andreea
AU - Szymczak, Wilfried
AU - Rink, Nadine
AU - Fuchs, Helmut
AU - Gailus-Durner, Valerie
AU - Wurst, Wolfgang
AU - Hoeschen, Christoph
AU - Klingenspor, Martin
AU - Hrabě De Angelis, Martin
AU - Rozman, Jan
N1 - Publisher Copyright:
© 2016 IOP Publishing Ltd.
PY - 2016/2/10
Y1 - 2016/2/10
N2 - The prevalence of obesity is still rising in many countries, resulting in an increased risk of associated metabolic diseases. In this study we aimed to describe the volatile organic compound (VOC) patterns symptomatic for obesity. We analyzed high fat diet (HFD) induced obese and mono-genetic obese mice (global knock-in mutation in melanocortin-4 receptor MC4R-ki). The source strengths of 208 VOCs were analyzed in ad libitum fed mice and after overnight food restriction. Volatiles relevant for a random forest-based separation of obese mice were detected (26 in MC4R-ki, 22 in HFD mice). Eight volatiles were found to be important in both obesity models. Interestingly, by creating a partial correlation network of the volatile metabolites, the chemical and metabolic origins of several volatiles were identified. HFD-induced obese mice showed an elevation in the ketone body acetone and acrolein, a marker of lipid peroxidation, and several unidentified volatiles. In MC4R-ki mice, several yet-unidentified VOCs were found to be altered. Remarkably, the pheromone (methylthio)methanethiol was found to be reduced, linking metabolic dysfunction and reproduction. The signature of volatile metabolites can be instrumental in identifying and monitoring metabolic disease states, as shown in the screening of the two obese mouse models in this study. Our findings show the potential of breath gas analysis to non-invasively assess metabolic alterations for personalized diagnosis.
AB - The prevalence of obesity is still rising in many countries, resulting in an increased risk of associated metabolic diseases. In this study we aimed to describe the volatile organic compound (VOC) patterns symptomatic for obesity. We analyzed high fat diet (HFD) induced obese and mono-genetic obese mice (global knock-in mutation in melanocortin-4 receptor MC4R-ki). The source strengths of 208 VOCs were analyzed in ad libitum fed mice and after overnight food restriction. Volatiles relevant for a random forest-based separation of obese mice were detected (26 in MC4R-ki, 22 in HFD mice). Eight volatiles were found to be important in both obesity models. Interestingly, by creating a partial correlation network of the volatile metabolites, the chemical and metabolic origins of several volatiles were identified. HFD-induced obese mice showed an elevation in the ketone body acetone and acrolein, a marker of lipid peroxidation, and several unidentified volatiles. In MC4R-ki mice, several yet-unidentified VOCs were found to be altered. Remarkably, the pheromone (methylthio)methanethiol was found to be reduced, linking metabolic dysfunction and reproduction. The signature of volatile metabolites can be instrumental in identifying and monitoring metabolic disease states, as shown in the screening of the two obese mouse models in this study. Our findings show the potential of breath gas analysis to non-invasively assess metabolic alterations for personalized diagnosis.
KW - Gaussian graphical model
KW - high-fat diet
KW - melanocortin 4 receptor
KW - mouse pheromone
KW - non-invasive metabolic phenotyping
KW - volatile organic compound
UR - http://www.scopus.com/inward/record.url?scp=85001849999&partnerID=8YFLogxK
U2 - 10.1088/1752-7155/10/1/016009
DO - 10.1088/1752-7155/10/1/016009
M3 - Article
C2 - 26860833
AN - SCOPUS:85001849999
SN - 1752-7155
VL - 10
JO - Journal of Breath Research
JF - Journal of Breath Research
IS - 1
M1 - 016009
ER -