Die Simulation quantitativer Merkmale durch Gene mit biochemisch definierbarer Wirkung - VIII. Untersuchungen über das Absorptionsverhalten der Anthocyane

With reference to the concentration of aglycones in carefully hydrolysed anthocyanin extracts of 16 genotypes of Matthiola incana R.Br. it was investigated whether the measurement of the optical density of qualitatively different anthocyanin extracts may pass as a suitable measurement of the total content of anthocyanin pigments. - The results obtained after acid hydrolysis of different anthocyanin extracts show that the qualitative change of the pigment pattern influences in some cases the extinction values considerably. Thus, 3-mono-sides and 3-biosides were underestimated, whereas 3, 5-glycosides were overestimated. The acylation of the anthocyanin molecule seems to have a detectable influence only on the 3, 5-triglycosides. - The results obtained could be confirmed largely by comparison with purchased preparations and are in accordance with the coefficients of extinction we measured and those found in literature. From these results correction factors may be derived which are suited to transforming the extinction values obtained by measurements of the optical density to the same scale. - The influence of this transformation on the distribution of measurements and on the estimates of the parameters of the genes involved was examined by means of a formerly investigated trifactorial system.