Di-copper(ii) DNA G-quadruplexes as EPR distance rulers

David M. Engelhard; Andreas Meyer; Andreas Berndhäuser; Olav Schiemann; Guido H. Clever

doi:10.1039/c8cc04053b

Di-copper(ii) DNA G-quadruplexes as EPR distance rulers

David M. Engelhard, Andreas Meyer, Andreas Berndhäuser, Olav Schiemann, Guido H. Clever

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Artificial metal-base tetrads composed of square-planar Cu^II(pyridine)₄ complexes were covalently attached to both the 3′ and 5′ ends of tetramolecular DNA G-quadruplexes [Ld(G_3-5)LdT]₄ (L = pyridine ligand) of different lengths. Owing to the planar four-point attachment of the metal complexes, the magnetic orbitals (dx²-y²) of the d⁹-configured Cu^II cations are placed in a coplanar fashion, separated by the stacked guanine tetrads. Pulsed EPR-derived Cu^II-Cu^II distances and angles were found to be in agreement with those obtained from molecular dynamics simulations. DNA-confined transition metal spin labels open new ways to study oligonucleotide structure and DNA-protein complexes.

Original language	English
Pages (from-to)	7455-7458
Number of pages	4
Journal	Chemical Communications
Volume	54
Issue number	54
DOIs	https://doi.org/10.1039/c8cc04053b
State	Published - 2018
Externally published	Yes

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abstract = "Artificial metal-base tetrads composed of square-planar CuII(pyridine)4 complexes were covalently attached to both the 3′ and 5′ ends of tetramolecular DNA G-quadruplexes [Ld(G3-5)LdT]4 (L = pyridine ligand) of different lengths. Owing to the planar four-point attachment of the metal complexes, the magnetic orbitals (dx2-y2) of the d9-configured CuII cations are placed in a coplanar fashion, separated by the stacked guanine tetrads. Pulsed EPR-derived CuII-CuII distances and angles were found to be in agreement with those obtained from molecular dynamics simulations. DNA-confined transition metal spin labels open new ways to study oligonucleotide structure and DNA-protein complexes.",

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T1 - Di-copper(ii) DNA G-quadruplexes as EPR distance rulers

AU - Engelhard, David M.

AU - Meyer, Andreas

AU - Berndhäuser, Andreas

AU - Schiemann, Olav

AU - Clever, Guido H.

PY - 2018

Y1 - 2018

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AB - Artificial metal-base tetrads composed of square-planar CuII(pyridine)4 complexes were covalently attached to both the 3′ and 5′ ends of tetramolecular DNA G-quadruplexes [Ld(G3-5)LdT]4 (L = pyridine ligand) of different lengths. Owing to the planar four-point attachment of the metal complexes, the magnetic orbitals (dx2-y2) of the d9-configured CuII cations are placed in a coplanar fashion, separated by the stacked guanine tetrads. Pulsed EPR-derived CuII-CuII distances and angles were found to be in agreement with those obtained from molecular dynamics simulations. DNA-confined transition metal spin labels open new ways to study oligonucleotide structure and DNA-protein complexes.

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Di-copper(ii) DNA G-quadruplexes as EPR distance rulers

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