Abstract
An enzyme immunoassay was developed for the simultaneous detection of different pyrethroid insecticides. Several polyclonal antisera were raised in rabbits using 1R trans-permethric acid coupled to bovine serum albumin (BSA) as immunogen. Antiserum D12 recognized permethrin, cyfluthrin, cypermethrin, S-bioallethrin and the metabolite 1R trans-permethric acid with similar affinities. 50 % inhibition (50 % B/Bo) for permethrin occurred at 8| Hg/L; linearity ranged between 0.4 and 74 pg/L. The antisera D5 and D7 were obtained from preceding blood samples, and the antibodies showed different cross-reactivities, revealing the path of affinity maturation of the antiserum.
| Original language | English |
|---|---|
| Pages (from-to) | 781-795 |
| Number of pages | 15 |
| Journal | Analytical Letters |
| Volume | 28 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1995 |
Keywords
- allethrin S-bioallethrin cyfluthrin cypermethrin. enzyme immunoassay
- insecticide pyrethroid permethrin polyclonal antibody _ stereoselectivity