Development of an enzyme-linked immunosorbent assay (ELISA) for pyrene

Klaus Meisenecker, Dietmar Knopp, Reinhard Niessner

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

An indirect competitive enzyme-linked immunosorbent assay (ELISA) for the polycyclic aromatic hydrocarbon pyrene has been developed. Polyclonal antibodies were raised in rabbits after immunization with a 1-pyrenylbutyric acid-keyhole limpet hemocyanin conjugate. A 1-aminopyrine-bovine serum albumin conjugate was prepared as coating antigen. The assay had a linear working range from 0.09 to 50 ppb with a centre point value (50% inhibition) of 0.58 ppb and a lower detection limit of 0.04 ppb in water. The mean intra-assay and interassay coefficients of variation were less than 7% over the range of the standard curve. Organic solvents tested so far interfered in the ELISA at final concentrations of 5% and greater. With acetonitrile and dimethyl sulfoxide a shift of the centre point value of the standard curve to about 7 and 20 ppb, i.e. a decreased sensitivity was found.

Original languageEnglish
Pages (from-to)114-118
Number of pages5
JournalAnalytical methods & instrumentation
Volume1
Issue number2
StatePublished - 1993

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