Development of an enzyme immunoassay for the determination of the herbicide metsulfuron-methyl based on chicken egg yolk antibodies

E. Welzig; H. Pichler; R. Krska; D. Knopp; R. Niessner

doi:10.1080/03067310008041347

Development of an enzyme immunoassay for the determination of the herbicide metsulfuron-methyl based on chicken egg yolk antibodies

E. Welzig, H. Pichler, R. Krska, D. Knopp, R. Niessner

Center for Analytical Chemistry, University of Applied Life Sciences and Natural Resources, Department IFA-Tulln

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The development of an indirect competitive enzyme immunoassay for the sulfonylurea herbicide metsulfuron-methyl (MSM) is described. In contrast to traditional antibody generation in mammals, this extremely sensitive method is based on chicken egg yolk antibodies (IgY). They were raised in laying hens using an MSM-derivative-BSA hapten as immunogen. With a 1:10000 dilution of the antibody solution and a coating antigen (MSM-derivative-KLH) concentration of 10 μg L^-1 the IC₅₀ value achieved for the target analyte was 0.4 μg L^-1. The least detectable dose was established at 13 ng L^-1. Cross-reactivity was tested with 5 structurally related compounds, where only sulfometuron showed a significant binding. The ELISA was tested with spiked tap and surface water samples. This paper, for the first time, demonstrates the production of high-affinity IgY antibodies for a herbicide compound.

Original language	English
Pages (from-to)	279-288
Number of pages	10
Journal	International Journal of Environmental Analytical Chemistry
Volume	78
Issue number	3-4
DOIs	https://doi.org/10.1080/03067310008041347
State	Published - 2000
Externally published	Yes

Keywords

ELISA
Egg yolk antibodies
Herbicide
IgY
Metsulfuron-methyl

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10.1080/03067310008041347

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title = "Development of an enzyme immunoassay for the determination of the herbicide metsulfuron-methyl based on chicken egg yolk antibodies",

abstract = "The development of an indirect competitive enzyme immunoassay for the sulfonylurea herbicide metsulfuron-methyl (MSM) is described. In contrast to traditional antibody generation in mammals, this extremely sensitive method is based on chicken egg yolk antibodies (IgY). They were raised in laying hens using an MSM-derivative-BSA hapten as immunogen. With a 1:10000 dilution of the antibody solution and a coating antigen (MSM-derivative-KLH) concentration of 10 μg L-1 the IC50 value achieved for the target analyte was 0.4 μg L-1. The least detectable dose was established at 13 ng L-1. Cross-reactivity was tested with 5 structurally related compounds, where only sulfometuron showed a significant binding. The ELISA was tested with spiked tap and surface water samples. This paper, for the first time, demonstrates the production of high-affinity IgY antibodies for a herbicide compound.",

keywords = "ELISA, Egg yolk antibodies, Herbicide, IgY, Metsulfuron-methyl",

author = "E. Welzig and H. Pichler and R. Krska and D. Knopp and R. Niessner",

note = "Funding Information: This study was financially supported by the Jubilaumsfond der Osterreichischen Nationalbank (No. 6571). The authors are grateful to Dr. Marcela Hermann and her team for their excellent animal care at the Institute for Molecular Genetics of the University of Vienna.",

year = "2000",

doi = "10.1080/03067310008041347",

language = "English",

volume = "78",

pages = "279--288",

journal = "International Journal of Environmental Analytical Chemistry",

issn = "0306-7319",

publisher = "Taylor and Francis Ltd.",

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T1 - Development of an enzyme immunoassay for the determination of the herbicide metsulfuron-methyl based on chicken egg yolk antibodies

AU - Welzig, E.

AU - Pichler, H.

AU - Krska, R.

AU - Knopp, D.

AU - Niessner, R.

N1 - Funding Information: This study was financially supported by the Jubilaumsfond der Osterreichischen Nationalbank (No. 6571). The authors are grateful to Dr. Marcela Hermann and her team for their excellent animal care at the Institute for Molecular Genetics of the University of Vienna.

PY - 2000

Y1 - 2000

N2 - The development of an indirect competitive enzyme immunoassay for the sulfonylurea herbicide metsulfuron-methyl (MSM) is described. In contrast to traditional antibody generation in mammals, this extremely sensitive method is based on chicken egg yolk antibodies (IgY). They were raised in laying hens using an MSM-derivative-BSA hapten as immunogen. With a 1:10000 dilution of the antibody solution and a coating antigen (MSM-derivative-KLH) concentration of 10 μg L-1 the IC50 value achieved for the target analyte was 0.4 μg L-1. The least detectable dose was established at 13 ng L-1. Cross-reactivity was tested with 5 structurally related compounds, where only sulfometuron showed a significant binding. The ELISA was tested with spiked tap and surface water samples. This paper, for the first time, demonstrates the production of high-affinity IgY antibodies for a herbicide compound.

AB - The development of an indirect competitive enzyme immunoassay for the sulfonylurea herbicide metsulfuron-methyl (MSM) is described. In contrast to traditional antibody generation in mammals, this extremely sensitive method is based on chicken egg yolk antibodies (IgY). They were raised in laying hens using an MSM-derivative-BSA hapten as immunogen. With a 1:10000 dilution of the antibody solution and a coating antigen (MSM-derivative-KLH) concentration of 10 μg L-1 the IC50 value achieved for the target analyte was 0.4 μg L-1. The least detectable dose was established at 13 ng L-1. Cross-reactivity was tested with 5 structurally related compounds, where only sulfometuron showed a significant binding. The ELISA was tested with spiked tap and surface water samples. This paper, for the first time, demonstrates the production of high-affinity IgY antibodies for a herbicide compound.

KW - ELISA

KW - Egg yolk antibodies

KW - Herbicide

KW - IgY

KW - Metsulfuron-methyl

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DO - 10.1080/03067310008041347

M3 - Article

AN - SCOPUS:0034451613

SN - 0306-7319

VL - 78

SP - 279

EP - 288

JO - International Journal of Environmental Analytical Chemistry

JF - International Journal of Environmental Analytical Chemistry

IS - 3-4

ER -

Development of an enzyme immunoassay for the determination of the herbicide metsulfuron-methyl based on chicken egg yolk antibodies

Abstract

Keywords

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